不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究     

李洁  杨静  赵丽丽 《武汉体育学院学报》2019,53(5):88-94

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。  相似文献   

运动中的酸碱平衡与碱盐摄入     

王晨 《体育科研》2003,24(4):60-61

采用文献资料法对人体酸碱平衡机制进行分析研究,并对碱盐摄入与对运动员成绩的影响进行了探讨,结果认为适当碱盐摄入对提高运动成绩有帮助.  相似文献   

透明质酸与运动能力     

张新辉  熊正英 《辽宁体育科技》2006,28(5):20-21

分析了透明质酸的多种生物学功能,尤其在保水、清除自由基、运动损伤治疗和增加运动免疫方面的功能,探讨其提高运动能力的生物学机制,并对其作为运动营养补剂的发展前景给予展望。  相似文献   

试论南京国民政府对我国盐政主权的收回     

姚顺东 《哈尔滨学院学报》2004,25(5):44-47

南京国民政府成立后，在关税自主、恢复和保留盐务稽核所的同时，通过变更职权和对洋人任用的变更开始了收回我国盐政主权的活动。南京国民政府当时对我国盐政主权的收回活动，现在看来，具有一定的历史意义。  相似文献   

男子400m运动员间歇训练的实验研究   总被引：4，自引：0，他引：4  

张永虎  郝立军  刘彬 《浙江体育科学》2002,24(1):18-20

通过严格实施 30 0m× 4m(次间间歇递减 )× 3组 (组间间歇递增 )的间歇跑和分析运行员实验前后运动成绩、心率恢复指数、血乳酸的变化 ,结果表明 :次间间歇递减和组间间歇递增更能深度刺激机体抗乳酸能力 ,对 40 0m运动员速度耐力有较大影响。旨在为提高我国男子 40 0m训练水平提供参考。  相似文献   

Environmentally-induced stone decay: the cumulative effects of crystallization–hydration cycles on a Lincolnshire oopelsparite limestone     

Belinda J. Colston  David S. Watt  Helen L. Munro   《Journal of Cultural Heritage》2001,2(4)

An investigation has been undertaken at St Andrew’s church, Walpole St Andrew, Norfolk, to establish the underlying causes of the observed stone decay to the upper parts of the six stone piers. The stone decay was first recorded in the early 1930s. The salt-contaminated masonry within the church has been shown to undergo severe salt decay during the summer, with little damage occurring over the winter months. The south aisle piers have been shown to decay 2.5 times faster than the north aisle piers. Although crystallization–hydration cycles have been identified, the rate of decay is at its greatest when the cycling is relatively infrequent. This was not the expected trend. Furthermore, it has been shown that during extended periods where the ambient relative humidity is less than 75%, the rate of decay reaches a maximum. It is the length of this ‘drying’ period that apparently has the greatest influence on the rate of decay and could explain the significant difference in the rate of decay between the south and north aisle piers. The results have serious implications for passive conservation, where it is often recommended to lower the ambient relative humidity to well below the equilibrium relative humidity of the salt contaminant, to avoid crystallization–hydration cycles. Since, at the time of building, the church was situated on the coast, it is possible that the sodium chloride contamination occurred during the building process (1440–1520), particularly since the area was prone to sea-flooding at this time. Alternatively, the salt could have been applied as a treatment during the general restoration of 1897. Whatever the source of the salt, it seems likely that the ambient environment was changed by the insertion of a sealed floor in 1897, which could account for the onset of the salt decay.  相似文献   

三元混配化合物的极谱研究--Phosphonic-、Amino-acid-Eu体系稳定性     

刘新文  卢小 《天水师范学院学报》2001,21(2):40-41

以脉冲极谱法研究了用有机磷酸和氨基酸作为配体与Eu(Ⅲ)形成两元及三元体系配合物的极谱行为.模拟生理条件,在μ=O.1(NaCl)、T=310±0.2K条件下,电极过程为准可逆;测定了配合物的稳定常数;讨论了复杂体系各配合物稳定性差异及其影响因素.  相似文献   

对高水平散手运动员赛前训练部分生化指标的监测分析   总被引：8，自引：2，他引：6  

谭欢  张育明  徐国栋 《武汉体育学院学报》2005,39(5):44-46,56

通过文献资料法、实验法、数理统计法和系统方法,对湖北省参加2004年全国散打锦标赛的高水平运动员在赛前训练的部分生化指标进行了监测。结论表明:通过对赛前血色素、血尿素、血清肌酸激酶变化的观察,其能反映高水平运动员对赛前运动负荷的适应情况以及身体机能的状况。安静时血乳酸值对周训练后运动员机能水平变化的反映不灵敏,运动后血乳酸值可反映赛前训练对抗强度及运动员的无氧能力。  相似文献   

Development of a chemically defined medium for Planctopirus limnophila to increase biomass production     

《Electronic Journal of Biotechnology》2021

BackgroundPlanctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production.ResultsWe found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner’s basal salts, 23.18 mM KNO₃, 2.318 mM NH₄Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final ΔOD₆₀₀ of 46.8 ± 0.5 after 108 h, corresponding to a cell dry weight of 13.6 ± 0.7 g/L.ConclusionsThe optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.How to cite: Kruppa OC, Gerlach D, Fan R, et al. Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.002.  相似文献   

玉米淀粉接枝高吸水性树脂的合成研究   总被引：1，自引：0，他引：1  

李雅丽  刘娟 《渭南师范学院学报》2003,18(5):34-35

对影响淀粉—丙烯酸接枝共聚反应的引发剂用量、淀粉／单体比例、聚合温度和聚合时间等因素进行了实验考察，确定了合成玉米淀粉接枝高吸水性树脂的最佳工艺条件。合成了吸水率为400g．g^-1～600g．g^-1的高吸水性树脂．为其工业化生产提供了技术参数。  相似文献