运动、氧化应激与DNA损伤和修复   总被引：4，自引：0，他引：4  

常波 《沈阳体育学院学报》2004,23(6):756-757,788

在分子和基因水平上对运动时氧化应激损伤、氧化应激与DNA损伤以及DNA损伤和修复等几方面关系进行探讨,旨在探讨运动疲劳和损伤发生的分子生物学基础,并对未来运动医学研究的发展趋势进行展望。  相似文献   

输煤控制系统硬件结构与上位机监控软件设计     

应力  张琳 《上海海事大学学报》2001,22(1):4-7

根据工况将不同的设备组合成不同的工作流程，以集散控制技术为基础，应用PLC网络实现了对输煤设备的现场控制、电控室控制和中控室控制。并以FIX软件为工具，通过定义PLC的I/O端口映射表方法，以时间、事件、故障为触发手段，设计了实时监控软件，并实现了输煤量与数据库系统的数据接口。  相似文献   

骨骼肌基因转移系统的建立     

陆爱云  文立  曾凡辉  陈立  邹蓓艳  卢大儒  薛京伦 《上海体育学院学报》2002,26(4):43-47

以EGFP为标记基因，利用重新构建的pAV-EGFP质粒，采用直接注射方法，进行了离体和在体基因转移研究。实验结果显示，经脂质体包裹后，质粒可以有效地进行细胞转移；DNA表达载体肌肉直接注射后，利用共聚焦显微镜可以在注射点周围的肌细胞观察到绿色荧光蛋白；而在对侧肢PCR和共聚焦显微镜的结果均为阴性。实验结果表明，肌肉直接注射DNA可以在肌肉得到表达，将会为治疗肌肉损伤带来新的方法。  相似文献   

运动性疲劳分子生物学机制的研究进展   总被引：12，自引：0，他引：12  

徐明  蔡国梁  李若愚 《成都体育学院学报》2005,31(4):91-94,98

从四个方面(细胞内分子、细胞膜分子、细胞外分子和其他广泛存在的分子和离子)综合分析运动性疲劳分子生物学机制的研究进展,以期达到完善运动性疲劳机制理论体系的目的.  相似文献   

浅谈巡视在工程监理中的作用     

朱礼俊  黄雄 《长江工程职业技术学院学报》2005,22(1):26-27

从质量、进度和投资三方面论述了监理巡视的作用和重要性.  相似文献   

Inclusive Learning: the Report of the Committee of Enquiry into the postschool education of those with learning difficulties and/or disabilities,in England, 1996     

John Tomlinson 《欧洲特需教育杂志》2013,28(3):184-196

ABSTRACT

The Report, Inclusive Learning (1996), is the result of a three‐year enquiry into the educational needs of and provision for adults with disabilities and/or learning difficulties in England. It arose from the requirement of an Act of Parliament, in 1992, that the new national funding council for further education should ‘have regard’ for such students in all its work of funding, development and evaluation. The Committee commissioned some fundamental research into the nature and extent of provision, the actual and potential demand for further education and the current requirements of law, and also reviewed existing academic research (chiefly in English‐speaking countries). It took extensive evidence in many modes and, in particular, created a series of workshops in which the students and their advocates could speak for themselves. The outcome was the main Report and summary, together with six supplementary publications. The core of the Report is the notion of ‘inclusive learning’, which places the responsibility for providing appropriate education with the teachers, the managers and the system (ultimately, with society), rather than problematizing the student as one with a deficit. A good education system is not merely about offering access to what is available, but also the making of what needs to be available accessible: the moulding of opportunity. Thus the Report concentrates on a theory of learning and its educational consequences. It represents another step towards embracing students with learning difficulties and/or disabilities unequivocally within the general approach to learning appropriate for all students. This article summarizes the main lines of analysis and argument in the Report and its framework of recommendations to the Further Education Funding Council, teachers, managers, voluntary organizations and to the government.  相似文献   

DNA生物大分子的动力学特性与生物功能(Ⅱ)     

庞小峰 《商丘师范学院学报》1996,(Z2)

对ＤＮＡ中激发的孤立子进行了分类，同时也研究了它的一些特点．我们着重研究了用此理论解释ＤＮＡ的基本生物功能──复制和转录及遗传与变异等问题．  相似文献   

盐浓度、酸碱度及DNA浓度对增色效应实验的影响   总被引：1，自引：0，他引：1  

曾碧慧  张志刚 《实验室研究与探索》2001,20(1):43-45,50

研究了DNA在不同浓度，不同pH值和不同氯化钠溶液中紫外吸光度的变化，为设计DNA的增色效应实验提供了依据。  相似文献   

芒果基因组DNA的提取方法比较   总被引：3，自引：0，他引：3       下载免费PDF全文

段中岗  庄海波  张嘉铭 《惠州学院学报》2006,26(3):15-17

采用SDS法、改良SDS法、CTAB法、改良CTAB法提取芒果基因组DNA,并对提取的DNA进行质量比较。结果表明:改良CTAB法能较好地去除芒果叶片中的酚类和多糖杂质,提取的DNA母液浓度达767．2 ng/μl, OD260／OD280为1．89,DNA纯度和浓度在四种方法中均为最高。  相似文献   

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant     

陈建忠  朱海红  刘克洲  陈智 《浙江大学学报(A卷英文版)》2004,5(4):467-471

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.  相似文献