长期运动训练对运动性低血色素大鼠组织转铁蛋白及转铁蛋白受体基因表达的影响   总被引：2，自引：0，他引：2  

曹建民  田野  赵杰修  郑弘溶  金丽  赵宁宁 《北京体育大学学报》2006,29(11):1492-1494

了解发生运动性低血色素时转铁蛋白及转铁蛋白受体基因表达的变化对深入研究运动性贫血的发生机制具有重要的意义.实验结果表明:长期运动训练导致运动性低血色素大鼠肌肉组织中转铁蛋白和转铁蛋白受体基因表达均显著增加,表明运动训练造成的运动性低血色素大鼠肌肉中铁代谢紊乱,肌肉应激性使转铁蛋白以及转铁蛋白受体表达增加,以促进肌肉对铁的吸收;运动性低血色素大鼠肝脏组织中转铁蛋白基因表达均显著下降,这可能是由于肝脏铁储量增加而导致的转铁蛋白基因表达的适应性的降低有关;运动训练导致运动性低血色素大鼠转铁蛋白和转铁蛋白受体表达具有组织特异性,转铁蛋白受体基因表达与组织铁代谢关系密切.  相似文献   

Advances in the phylogenesis of Agaricales and its higher ranks and strategies for establishing phylogenetic hypotheses     

Zhao RL  Desjardin DE  Soytong K  Hyde KD 《Journal of Zhejiang University. Science. B》2008,9(10):779-786

We present an overview of previous research results on the molecular phylogenetic analyses in Agaricales and its higher ranks (Agaricomycetes/Agaricomycotina/Basidiomycota) along with the most recent treatments of taxonomic systems in these taxa. Establishing phylogenetic hypotheses using DNA sequences, from which an understanding of the natural evolutionary relationships amongst clades may be derived, requires a robust dataset. It has been recognized that single-gene phylogenies may not truly represent organismal phylogenies, but the concordant phylogenetic genealogies from multiple-gene datasets can resolve this problem. The genes commonly used in mushroom phylogenetic research are summarized.  相似文献   

1016名学生PTC尝味能力基因频率的调查     

刘继云  杨春玫  孙建永  郭建梅 《河北北方学院学报(医学版)》2001,(1)

目的:了解味盲基因频率和基因型频率在不同群体中的分布,从而为人种学及人类群体遗传学提供基本数据,并用于研究某些疾病。方法:本文是运用阈值法研究满、汉、回三民族人的尝味能力,并计算了味盲者和尝味者的基因频率。结果:三民族味盲基因频率分别为:满族:0.4588.汉族:0.2845 回族:0.4431。结论:满、汉、回三民族人对PTC尝味能力在统计学上存在显著差异,不同的民族有不同的基因库。  相似文献   

L-ficolin突变体原核表达载体的构建及表达产物的鉴定     

张家忠  李小燕  向田  章晓联 《襄樊职业技术学院学报》2007,6(2):23-26

目的构建L-ficolin突变体的原核表达载体，为研究L-ficolin的糖结合位点奠定基础。方法设计L-ficolin突变体基因上、下游引物，分别引入EcoRⅠ和XhoⅠ酶切位点。以插入L—ficolin M2（Val144Phe）和M6（Glu 307Asp）点突变的完整编码区基因的质粒pCDNA3-L-ficolin为模板，通过PCR扩增获得突变体的基因片段，将其克隆入原核表达载体pGEX—KG．然后通过酶切和序列测定对其进行鉴定；将此表达载体转化入表达菌BL21（DE3）pLvSs诱导表达。并采用SDS—PAGE对表达产物进行鉴定。结果经酶切及序列分析表明．成功地构建了重组原核表达载体pGEX-KG—L—ficolin—M2、pGEX-KG-L-ficolin—M6，SDS—PAGE显示目的蛋白大量表达。结论L—ficolin突变体融合蛋白可以在大肠杆菌中大量表达。为研究L-ficolin的糖结合位点打下基础。  相似文献   

Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population     

Yang YM  Xu ZR  Wu LJ  Huang WD 《Journal of Zhejiang University. Science. B》2007,8(2):132-135

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…  相似文献   

耐力素质与力量素质相关基因的研究进展   总被引：1，自引：0，他引：1  

邓文骞  王璐  左天香  王玉 《四川体育科学》2007,(1):34-37,41

研究表明,运动能力在很大程度上受到遗传因素的影响,人类有多个基因与人体运动能力密切相关。本文运用文献资料法,综述了近年来国内外关于耐力与力量素质相关基因的研究进展,初步探讨了这些基因影响运动能力的途径,为接下来在该领域的研究提供了参考和建议。  相似文献   

Polyethylenimine-cyclodextrin-tegafur conjugate shows anti-cancer activity and a potential for gene delivery     

Hu QD  Fan H  Lou WJ  Wang QQ  Tang GP 《Journal of Zhejiang University. Science. B》2011,12(9):720-729

Polyethylenimine-cyclodextrin-tegafur (PEI-CyD-tegafur) conjugate was synthesized as a novel multifunctional prodrug of tegafur for co-delivery of chemotherapeutic agent tegafur and enhanced green fluorescent protein (EGFP) reporter plasmid DNA. Conjugation of tegafur to PEI-CyD via chemical linkage was characterized by ¹H NMR spectrometry and ultraviolet (UV) spectrometry. PEI-CyD-tegafur was able to condense plasmid DNA into complexes of around 150 nm with positive charge at the N/P ratio of 25, in accordance with electron microscopy observation of compact and monodisperse nanoparticles. The results of in vitro experiments showed enhanced cytotoxicity and considerable transfection efficiency in B16F10 cell line. Therefore, PEI-CyD-tegafur may have great potential as a co-delivery system with anti-cancer activity and potential for gene delivery.  相似文献   

RRM1 gene expression in peripheral blood is predictive of shorter survival in Chinese patients with advanced non-small-cell lung cancer treated by gemcitabine and platinum     

Wang LR  Zhang GB  Chen J  Li J  Li MW  Xu N  Wang Y  Shen Tu JZ 《Journal of Zhejiang University. Science. B》2011,12(3):174-179

Objective  

To evaluate the predictive values of gene expressions of ribonucleotide reductase M1 (RRM1) and breast cancer susceptibility gene 1 (BRCA1) in peripheral blood from Chinese patients with non-small-cell lung cancer (NSCLC) treated with gemcitabine plus platinum.  相似文献   

DNA标记及其在鱼类中的应用     

肖明松  鲍方印  夏海武  崔峰 《安徽科技学院学报》2011,25(5):10-15

DNA分子标记是继形态标记、细胞标记和生化标记之后发展起来的一种比较理想的遗传标记技术。本文简要介绍RFLP、RAPD、AFLP、SSR、SNP、EST、mtDNA等DNA分子标记的类型,基本原理和特点,以及分子标记技术在鱼类遗传图谱构建、基因定位和分子标记辅助育种等方面的应用前景予以展望。  相似文献   

Aroma changes of black tea prepared from methyl jasmonate treated tea plants   总被引：1，自引：0，他引：1  

Jiang SHI  Li WANG  Cheng-ying MA  Hai-peng LV  Zong-mao CHEN  Zhi LIN 《Journal of Zhejiang University. Science. B》2014,15(4):313-321

研究目的：为茉莉酸甲酯诱导来提高茶叶香气品质提供理论依据,为新型花香红茶的研制提供技术支持。创新要点：首次将茉莉酸甲酯应用于诱导茶叶香气品质提高,初步验证了茶叶香气品质提高的本质原因：相关酶活性提高,基因表达上调。研究方法：采用顶空固相微萃取法（HS-SPME）对红茶香气进行富集,气相色谱-质谱联用仪（GC-MS）进行解吸附分析,实时定量多聚酶链式反应（qRT-PCR）分析茶鲜叶中香气相关酶基因表达。重要结论：茉莉酸甲酯诱导后的茶鲜叶中多酚氧化酶（PPO）活性上升,β-葡萄糖苷酶活性下降;PPO和β-樱草糖苷酶基因表达上调,β-葡萄糖苷酶基因表达下调。茉莉酸甲酯诱导后的茶鲜叶能明显提高由其制成的红茶香气品质,且萜烯醇类和萜烯类含量明显提高。  相似文献