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1.
Induction of diabetes by Streptozotocin in rats 总被引：1，自引：0，他引：1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
2.
Diabetes Mellitus in obese and non-obese Indian individuals.AIMS: Effect of Obesity and insulin resistance on diabetic control.SETTINGS AND DESIGN: 50 each groups Diabetic individuals obese and non-obese.METHODS AND MATERIAL: On selected 50 each group diabetic patient and normal, following blood investigations has been performed—Plasma Glucose,
Glycohemoglobin and Serum Insulin.STATISTICAL ANALYSIS USED: Individuals patient’s results were analyzed and compared with the normal controls.RESULTS AND CONCLUSION: The changes in glycosylated haemoglobin are mainly proportional to the post lunch glucose level (r=0.773) (p<0.01) and not
correlated to circulating insulin or the body mass index. However the levels were higher in obese diabetes (Type I and II
both) than in non-obese. Mechanism of resistance in insulin receptor interactions due to obesity is well known. However, obesity
does not seem to affect directly glycosylated haemoglobin. Under such circumstances, the reduction of weight for a diabetic
person can improve sugar control by minimizing insulin resistance and thereby can improve glycosylated haemoglobin levels. 相似文献
3.
BackgroundDiabetes is a metabolic disorder caused by defects in insulin production and activity. During disease progression, changes in lipid peroxidation cause structural modifications via production of free radicals. Fangchinoline is a well-known alkaloid present in Stephaniae tetrandrine S. Moore, which has demonstrated antioxidant, anticancer, and anti-inflammatory activities.ResultsThe present study analyzed the anti-diabetic and antioxidant effects of fangchinoline in male rats with streptozotocin-induced diabetes. Rats were divided into the following groups: normal control, diabetic, diabetic + fangchinoline 100 mg/kg, diabetic + fangchinoline 200 mg/kg and diabetic + glibenclamide 600 µg/kg. The treatment was administered orally for 45 consecutive days. Lipid peroxidation was substantially increased by >50% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. However, fangchinoline supplementation significantly reduced lipid peroxidation to near normal levels. Reactive oxygen species levels were substantially increased by >500% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. Fangchinoline supplementation reduced reactive oxygen species to near normal levels. Fangchinoline supplementation significantly improved superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels in diabetic rats. Total hexoses, sialic acid, hexosamines, and fucose were increased in diabetic rats, whereas fangchinoline supplementation significantly reduced these total hexoses, sialic acid, hexosamines, and fucose to near normal levelsConclusionsSupplementation with fangchinoline led to significant attenuation of the levels of lipid peroxidation, ROS, and glycoprotein components such as total hexoses, hexosamines, sialic acid, and fucose, while improving antioxidant marker levels.How to cite: Xia J, Huang W, Zhou F. Effect of fangchinoline on oxidant status in male albino rats with streptozotocin-induced diabetes. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.07.005 相似文献
4.
Liliana C. Baptista Aristides M. Machado-Rodrigues Raul A. Martins 《European Journal of Sport Science》2017,17(6):794-804
The aim of this cohort study is to analyse the effect of three types of treatment: (i) exercise training with multicomponent exercise (E); (ii) pharmacologic treatment with oral hypoglycaemic drug – metformin (M); and (iii) a combined therapy – exercise and metformin (E?+?M) on health-related quality of life (HRQoL) and mood states in older adults with type 2 diabetes (T2D) with comorbidity in an early stage of the disease. Participants (n?=?284) underwent 1 of the following 3 conditions: (i) E (n?=?59) trained three times/week; (ii) M (n?=?30) used 850?mg of metformin twice daily; and (iii) E?+?M (n?=?195) combined exercise and metformin. Furthermore, participants completed baseline and 2-year follow-up evaluations including a Shortform Health Survey 36, Profile of Mood States – Short-form, the health history questionnaires, anthropometric, and blood biochemistry. E and E?+?M revealed improved mood states, with large effect size on the vigour domain, and moderate effect size in the anger and total mood disturbance (TMD) domains (P?<?0.05), in comparison with the M group. After 24 months’ intervention, the E and E?+?M groups perceived better physical and mental HRQoL than the M group. The M group unchanged HRQoL domains (P?>?0.05). Metformin had no significant effect on the self-referred HRQoL in T2D participants aged above 60 years, in an early stage of the disease. The E and E?+?M were the most effective long-term therapies to improve mood states and HRQoL in older adults with T2D. 相似文献
5.
观察有氧运动对糖尿病模型大鼠血糖等生化指标的影响。四氧嘧啶诱导的糖尿病大鼠20只,随机分为实验组（10只）和对照组（10只）。实验组动物进行45d连续跑台运动,第46d断头取血测血糖、转氨酶、尿素氮和血常规指标。结果发现：有氧运动对糖尿病大鼠的BUN、MCV、MCH、MCHC无显著性（p〉0.05）影响;但与对照组相比,血糖、GPT、RBC、HCT、HGB、RDW、PLT、WBC却发生了显著性（p〈0.05）变化。 相似文献
6.
R. Dhananjayan T. Malati Y. Rupasree Vijay Kumar Kutala 《Indian journal of clinical biochemistry : IJCB》2015,30(3):263-270
The present work was aimed to study the association of one carbon genetic variants, hyperhomocysteinemia and oxidative stress markers, i.e., serum nitrite, plasma malondialdehyde (MDA) and glutathione (GSH) on intimal medial thickening (IMT) in patients with type 2 diabetes mellitus (T2D). A total number of 76 subjects from ACS Medical College and Hospital, Chennai, India were included in the study, i.e., Group I (n = 42) of T2D and Group II (n = 34) of age- and sex matched healthy controls. The glycated haemoglobin was measured by ion-exchange resin method; plasma homocysteine by Enzyme Linked Immunosorbant Assay method; serum nitrite (nitric oxide, NO), plasma MDA and GSH by spectrophotometric methods; the IMT by high frequency ultrasound. The polymorphisms of one carbon genetic variants were genotyped using polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism methods. Results indicate that methyltetrahydrofolate homocysteine methyl transferase (MTR) A2756G allele was found to be protective in T2D and the other variants were not significantly associated with T2D. Glutamate carboxypeptidase II (GCP II) C1561T (r = 0.34; p = 0.05) and methylene tetrahydrofolate reductase (MTHFR) C677T (r = 0.35; 0.04) showed positive correlation with plasma homocysteine in T2D cases. In this study, MTR A2756G allele was found to be protective in T2D; GCP II C1561T and MTHFR C677T showed positive association with plasma homocysteine in T2D cases. Among all the genetic variants, MTR A2756G was found influence IMT. RFC 1 G80A and TYMS 5′-UTR 2R3R showed synergistically interact with MTR A2756G in influencing increase in IMT. 相似文献
7.
Kirtimaan Syal Anand Srinivasan Dibyajyoti Banerjee 《Indian journal of clinical biochemistry : IJCB》2015,30(3):323-328
Diabetes and tuberculosis are world’s most deadly epidemics. People suffering from diabetes are susceptible to tuberculosis. Molecular link between the two is largely unknown. It is known that Vitamin A receptor (RXR) heterodimerizes with Vitamin D receptor (VDR) and Peroxisome proliferator-activator receptor-γ (PPARγ) to regulate Tryptophan-aspartate containing coat protein (TACO) expression and fatty acid metabolism respectively, so it would be interesting to check the expression of these genes in diabetes mellitus (DM) patients which might explain the susceptibility of diabetics to tuberculosis. In this study, we checked the expression of RXR, VDR, TACO and Interferon-γ (IFNγ) genes in type-2 DM patients for understanding the link between the two diseases. We observed down regulation of RXR gene and corresponding up regulation of TACO gene expression. We have not observed significant change in expression of VDR and IFNγ genes in type-2 DM patients. Repression of RXR gene could hamper VDR-RXR heterodimer formation and thus would up regulate TACO gene expression which may predispose the type-2 DM patients to tuberculosis. Also, decrease in RXR-PPARγ heterodimer could be involved in DM. 相似文献
8.
运动对GLUT4影响的研究进展 总被引：1，自引：0，他引：1
葡萄糖跨膜转运是机体利用葡萄糖的首要步骤。葡萄糖载体(GLUT)是细胞膜上介导葡萄糖跨膜转运的蛋白质,它们对机体的葡萄糖利用有重要意义。运动可有效增加外周组织(主要为骨骼肌和脂肪组织)细胞膜葡萄糖载体4(GLUT4)的数量,使细胞内GLUT4的囊泡数量增多,GLUT4mRNA的表达增强,有效地加快血糖的转移、吸收和利用,解除外周组织胰岛素抵抗,调节血糖平衡,对糖尿病人的治疗有积极的作用和意义。 相似文献
9.
目的:探讨体育锻炼对老年Ⅱ型糖尿病患者身体形态、身体素质、身体机能及动脉阶段弹性的影响。方法:根据体育锻炼问卷结果将受试者分为锻炼组(n=73)和非锻炼组(n=49)进行相关指标的观察。结果:锻炼组的形态、机能、素质类指标多数优于非锻炼组,锻炼组与不锻炼组相比,除体重和血压不具有统计学差异外,其余指标均具有显著性差异;锻炼组肱动脉—踝动脉脉搏波传导速度显著低于非锻炼组,但踝臂指数与非锻炼组无显著性差异。结论:经常参加体育锻炼可增强老年Ⅱ型糖尿病患者的体质,提高老年Ⅱ型糖尿病患者的动脉顺应性。 相似文献
10.
目的探讨有氧运动联合膳食干预对2型糖尿病大鼠骨骼肌AMPK活性和蛋白含量的影响。方法 6周龄雄性SD大鼠62只,随机抽取8只大鼠作为正常对照组(C组,n=8),喂以标准普通饲料。其余54只在喂饲高糖高脂膳食的基础上,腹腔注射小剂量的链脲佐菌素(STZ),建立2型糖尿病动物模型。然后将2型糖尿病大鼠随机分成4组:DM对照组(DM,n=9)、DM+运动锻炼组(DME,n=10)、DM+膳食控制组(DMD,n=10)、DM+运动锻炼+膳食控制组(DMED,n=10)。DM组大鼠继续喂饲高脂高糖饲料,不进行运动锻炼;运动锻炼采用每天进行60 min的无负重游泳运动,每周6次;膳食控制采用与DM组等量的标准普通饲料。12周后,检测各组大鼠FPG、FINS、骨骼肌中AMPK含量和活性。结果 (1)与C组相比,DM组大鼠FPG显著升高(P<0.01),FINS显著降低(P<0.01)。双因素方差分析显示,与DM组相比,DME组FPG显著性降(P<0.05),FINS虽有上升,但没有显著性差异(P>0.05);DMD组FINS显著升高(P<0.05),但FPG没有显著性变化(P>0.05);运动联合膳食控制对糖尿病大鼠FPG、FINS均无显著性交互作用。(2)与C组相比,DM组大鼠骨骼肌中AMPK含量和活性均显著性下降(P<0.01,P<0.05);双因素方差分析显示,与DM组相比,DME组骨骼肌中AMPK含量和活性均有显著升高(P<0.05、P<0.01)。DMD组骨骼肌中AMPK含量和活性均无显著性变化。运动联合膳食控制对糖尿病大鼠骨骼肌中AMPK含量和活性均无显著性交互作用。结论 (1)研究成功复制了2型糖尿病大鼠模型,而有氧运动锻炼对2型糖尿病大鼠具有显著的干预作用。(2)2型糖尿病大鼠骨骼肌中AMPK含量和活性显著降低,有氧运动锻炼不仅有效地提高2型糖尿病大鼠骨骼肌AMPK含量,而且可显著增加AMPK活性,对改善2型糖尿病机体的糖脂代谢具有非常重要的作用,但膳食控制对其作用不明显。 相似文献