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目的探讨自主运动调节tau蛋白激酶和tau蛋白磷酸酶的基因表达,抑制APP/PS1转基因小鼠海马tau蛋白过度磷酸化,进而改善学习记忆能力的分子机制。方法C57系APP/PS1转基因小鼠随机分为运动组(TE)与对照组(TC);C57系野生小鼠亦随机分为运动组(E)与对照组(C)。其中,TE组和E组小鼠从3月龄开始,给予16周的跑轮运动装置,TC组和C组小鼠不施加运动干预,作为相应对照组。Morris水迷宫实验检测APP/PS1转基因小鼠的空间学习记忆能力,Western Blot实验检测海马磷酸化tau蛋白(P-tau)水平,RT-PCR实验检测海马tau蛋白及tau蛋白激酶GSK-3β和CDK-5,tau蛋白磷酸酶PP2A和PP1 mRNA表达。结果(1)与TC组相比,TE组小鼠Morris水迷宫游泳潜伏期显著性降低(P<0.05),平台象限的游泳时间百分比显著性增加(P<0.05),穿越原平台位置的次数极显著性增加(P<0.01);(2)与TC组相比,TE组小鼠海马总tau蛋白mRNA表达显著下调(P<0.05),Ser202位点和Thr181位点P-tau蛋白表达均显著性下调(P<0.05);(3)与TC组相比,TE组小鼠海马tau蛋白磷酸化激酶GSK-3β(P<0.01)和CDK-5(P<0.05)mRNA表达显著性下调,tau蛋白磷酸酶PP2A(P<0.01)和PP1(P<0.01)mRNA表达极显著性下调。结论16周自主运动通过下调tau蛋白激酶表达、上调tau蛋白磷酸酶表达,抑制APP/PS1转基因小鼠海马tau蛋白的过度磷酸化,进而促进海马神经细胞功能,改善转基因小鼠学习记忆。说明自主运动可通过调节脑的分子生物学机制,促进认知功能的改善和提升,预防和缓解阿尔茨海默病。 相似文献
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大量研究发现AD患者脑神经纤维tau蛋白的过度磷酸化形成NFTs,和Aβ在脑内的沉积形成老年斑,是导致AD发病的重要机制。在查阅了大量国内外有关tau蛋白和Aβ及运动对二者影响的文献的基础上,对导致tau蛋白异常过度磷酸化和Aβ产生的机制进行阐述,并就运动训练对tau蛋白过度磷酸化和Aβ的影响及防治AD的可能机制进行探讨。研究发现运动训练抑制了AD实验动物脑内tau蛋白的异常过度磷酸化和Aβ的沉积,在运动人体科学领域为体育运动防治AD的研究提供了可行性依据,也为AD的防治提供了除药物治疗外的新方法。 相似文献
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Kyle A. Caudle 《Teaching Statistics》2018,40(3):98-107
When playing card games, how many times should a deck be shuffled in order to achieve randomness? This article shows how card shuffling can be used as a classroom exercise to reinforce construction and interpretation of confidence intervals. 相似文献
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In this ITEMS module, we provide a two‐part introduction to the topic of reliability from the perspective of classical test theory (CTT). In the first part, which is directed primarily at beginning learners, we review and build on the content presented in the original didactic ITEMS article by Traub and Rowley (1991). Specifically, we discuss the notion of reliability as an intuitive everyday concept to lay the foundation for its formalization as a reliability coefficient via the basic CTT model. We then walk through the step‐by‐step computation of key reliability indices and discuss the data collection conditions under which each is most suitable. In the second part, which is directed primarily at intermediary learners, we present a distribution‐centered perspective on the same content. We discuss the associated assumptions of various CTT models ranging from parallel to congeneric, and review how these affect the choice of reliability statistics. Throughout the module, we use a customized Excel workbook with sample data and basic data manipulation functionalities to illustrate the computation of individual statistics and to allow for structured independent exploration. In addition, we provide quiz questions with diagnostic feedback as well as short videos that walk through sample exercises within the workbook. 相似文献
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以南瓜、西葫芦、板栗瓜、姜柄瓜、黄瓜、香瓜6种果实引诱雌虫产卵结果可知,南瓜实蝇在姜柄瓜上引诱产卵最多(294粒)、次之为板栗瓜(37.67粒)、西葫芦(26.33粒)、香瓜(16.67粒)、南瓜(14.33粒)、而在黄瓜上引诱产卵最少(10.33粒).南瓜实蝇雌虫产卵喜欢在瓜肉面产卵.雌虫产卵易受果实伤口引诱,雌虫喜欢在人为损伤的伤口上产卵. 相似文献
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采用伪谱方法中的Ｃｈｅｂｙｓｈｅｖ－τ方法和配置点方法，分别研究了平面Ｐｏｉｓｅｕｉｌｅ流中扰动的非线性演化问题，并进行了比较．结果表明，在中性情况附近，配置点方法的精度要高于Ｃｈｅｂｙｓｈｅｖ－τ方法的精度． 相似文献
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摘要：目的：探讨有氧运动对APP/PS1/tau小鼠清醒状态海马CA1区theta时段gamma节律的影响以及对睡眠状态海马CA1区SWR时段低频gamma节律的影响。方法：清洁级6月龄APP/PS1/tau小鼠随机分为(AS) 、运动组(AE),C57BL/6J对照组小鼠随机分为(CS)、运动组(CE),AE组和CE组进行12周跑台运动,5 d/周,60 min/d,前 10 min运动负荷为12 m/min,后 50 min为15 m/min,跑台坡度为 0°。八臂迷宫检测小鼠行为学变化;多通道在体记录技术记录海马CA1区清醒状态和睡眠状态的神经电信号,MATLAB提取清醒状态theta时间段和睡眠状态的SWR时间段,多窗谱估计法进行时频分析和功率谱密度分析。结果：12周有氧运动可明显改善AS组的工作记忆和参考记忆,增加清醒状态海马CA1区theta时段的gamma能量和睡眠状态CA1区SWR时段的低频gamma能量。结论：有氧运动可改善AD模型神经网络状态,提高海马CA1区theta时段gamma能量和SWR时段低频gamma能量是其整体行为学改善的神经网络机制之一。 相似文献
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采用室内湿度配比的方法研究了空气和土壤湿度对南瓜实蝇存活的影响,结果表明土壤相对含水量和空气湿度等有关湿度方面的条件对南瓜实蝇蛹的羽化和成虫的存活有显著影响.相对含水量在20﹪-60﹪之间蛹的羽化率较高,当土壤相对含水量达到100﹪时,南瓜实蝇蛹不能羽化.1日龄蛹经干燥处理8d后羽化率降低至28.89﹪.空气湿度对成虫存活也有显著影响,随着干旱和高湿胁迫时间的延长,成虫的死亡速率逐渐加快,但是干旱条件下的抑制效果更显著. 相似文献
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Mark A. Scott John van der Kamp Geert J. P. Savelsbergh Raôul R.D. Oudejans Keith Davids 《Research quarterly for exercise and sport》2013,84(2):130-137
Abstract We investigated how perturbing optical information affects the guidance of an unfolding hitting action. Using monocular and binocular vision, six participants were required to hit a rectangular foam object, released from two different heights, under four different approach conditions, two with object rotation (to perturb the viewed surface area) and two without. Participants made more misses under monocular conditions, and the presence of rotation had a significant effect on the duration between the arm's peak velocity and its contact with the object. We conclude that binocular information contributes to the timing of interceptive tasks. In addition, the guidance of interceptive actions does not appear to be based solely on object expansion information picked up from the viewed surface area. 相似文献
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考虑一阶线性双曲方程的一种耗散Legendre tau方法,使用对偶技巧得到其在一定解光滑性条件下的最优收敛性估计。 相似文献
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