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1.
Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200-250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.  相似文献   

2.
The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression.  相似文献   

3.
目的：观察天麻素对癫痫大鼠海马Caspase-3表达的影响及其脑保护作用。方法：120只健康成年雄性Wistar大鼠随机分为对照组、PTZ（戊四氮）组、VPA（丙戊酸钠）组、Gb（天麻素大剂量）组和Gs（天麻素小剂量）组（n=24）。对照组和PTZ组分别以生理盐水（4mL/kg·d）灌胃;VPA组给予丙戊酸20mg．kg^-1,Gb组和Gs组分别给予天麻素200mg．kg^-1和100mg．kg^-1;每天1次,连续7天。造模第一天,除对照组大鼠外,余者均腹腔注射戊四氮75mg／kg,记录动物行为学变化。于致痫后12h、2d、5d和7d相应时间点取材,制备脑标本,免疫细胞化学术检测caspase-3表达。结果：致痫后12h,P11Z组Caspase-3有微量表达,其余各组几乎无表达;2—7d。PTZ组Caspase-3表达增加。与PTZ组比较,Gb组、Gs组及VPA组Caspase-3阳性表达降低,差异显著（P〈0．05）;与VPA组比较,Gb组和Gs组Caspase-3表达无显著差异（P〉0．05）。结论：天麻素能降低致痫大鼠海马神经元Caspase-3表达;可能通过抑制神经元凋亡发挥脑保护作用。  相似文献   

4.
The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx),and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2 g/kg). On the contrary, increased activities of liver GSH, SOD, GPx,CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepatoprotective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.  相似文献   

5.
Objective: To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods: The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results: The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions: Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.  相似文献   

6.
This study probed the protective effect of recombinant Lactobacillus plantarum against hydrogen peroxide(H_2O_2)-induced oxidative stress in human umbilical vein endothelial cells(HUVECs). We constructed a new functional L. plantarum(NC8-p SIP409-alr-angiotensin-converting enzyme inhibitory peptide(ACEIP)) with a double-gene-labeled non-resistant screen as an expression vector. A 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazolium bromide(MTT) colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-p SIP409-alr-ACEIP. Flow cytometry(FCM) was used to determine the apoptosis rate of HUVEC cells. Cysteinyl aspartate specific proteinase(caspase)-3/8/9 activity was also assayed and western blotting was used to determine protein expression of B-cell lymphoma 2(Bcl-2), Bcl-2-associated X protein(Bax), inducible nitric oxide synthase(i NOS), nicotinamide adenine dinucleotide phosphate oxidase 2(gp91 phox), angiotensin II(Ang II), and angiotensin-converting enzyme 2(ACE2), as well as corresponding indicators of oxidative stress, such as reactive oxygen species(ROS), mitochondrial membrane potential(MMP), malondialdehyde(MDA),and superoxide dismutase(SOD). NC8-p SIP409-alr-ACEIP attenuated H_2O_2-induced cell death, as determined by the MTT assay. NC8-p SIP409-alr-ACEIP reduced apoptosis of HUVEC cells by FCM. In addition, compared to the positive control, the oxidative stress index of the H_2O_2-induced HUVEC(Hy-HUVEC), which was pretreated by NC8-p SIP409-alr-ACEIP, i NOS,gp91 phox, MDA, and ROS, was decreased obviously; SOD expression level was increased; caspase-3 or-9 was decreased, but caspase-8 did not change; Bcl-2/Bax ratio was increased; permeability changes of mitochondria were inhibited; and loss of transmembrane potential was prevented. Expression of the hypertension-related protein(Ang II protein) in HUVEC cells protected by NC8-p SIP409-alr-ACEIP decreased and expression of ACE2 protein increased. These plantarum results suggested that NC8-p SIP409-alr-ACEIP protects against H_2O_2-induced injury in HUVEC cells. The mechanism for this effect is related to enhancement of antioxidant capacity and apoptosis.  相似文献   

7.
目的:观察黄芩甙注射液对四氯化碳(CCl4)致急性肝损伤小鼠肝抗氧化功能的影响。方法:50只小鼠随机分为5组,每组10只,分别为对照组、模型组、黄芩甙注射液低、中、高(0.25 g/kg、0.50 g/kg、1.00g/kg)剂量组。用CCl4制备小鼠急性肝损伤模型,测定小鼠体重变化,肝指数,肝超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,谷胱甘肽(GSH)及丙二醛(MDA)含量。结果:与对照组及模型组比较,黄芩甙各剂量组小鼠试验前后的体重及肝指数差异不显著(P>0.05)。与模型组比较,低剂量组小鼠肝SOD、GSH-Px活性及MDA含量差异不显著(P>0.05),而GSH含量显著升高(P<0.05);中、高剂量组SOD、GSH-Px活性极显著提高(P<0.01),GSH含量极显著升高(P<0.01),MDA含量极显著降低(P<0.01)。结论:黄芩甙能显著增强急性肝损伤小鼠肝SOD、GSH-Px活性,提高GSH含量,降低MDA含量,并表现正向的量效关系。  相似文献   

8.
Objective  The aim of this study was to test the protective effect of mesenchymal stem cells (MSCs) on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods  MSCs from Sprague-Dawley (SD) rats were separated and cultured. MSC medium was collected from MSCs cultured in serum-free Dulbecco’s modified eagle medium (DMEM) under hypoxia. Cultured cardiomyocytes from neonatal SD rats were exposed to hypoxia/reoxygenation (H/R) and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate (FITC), Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cytochrome C, apoptosis-induced factor (AIF), and caspase-3 was tested by Western blot analysis. Results  Our data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cytochrome C and AIF from mitochondria into the cytosol. Conclusion  MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner. Project supported by the National Natural Science Foundation of China (No. 30670868) and the Natural Science Foundation of Zhejiang Province, China (No. R206007)  相似文献   

9.
To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee’s index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P<0.01). Six miRNAs expressed significantly differently in the liver (P<0.05). Specifically, expression levels of miR-378b were significantly lower in the H group than in the N group (P<0.05). Compared with the normoxic sedentary group, hypoxic exercise training resulted in a lower ratio of FAS mRNA to CPT1A mRNA (P<0.05), as well as lower CPT1A protein levels (P<0.01), while a higher ratio of FAS to CPT1A protein levels (P<0.01) was observed. In conclusion, hypoxic training may elevate the resistance of high fat diet induced obesity in rats by reducing the expression of miR-378b, and decrease the fatty acid mitochondrial oxidation in obese rat livers by decreasing the protein expression of CPT1A and increasing the protein expression ratio of FAS/CPT1A.  相似文献   

10.
为了研究大豆黄酮对草杂鸡消化系统发育的影响。选用300只1日龄健康草杂鸡,分别随机分为5组,即Ⅰ组和4个试验组。Ⅰ组饲喂基本日粮,在Ⅱ,Ⅲ,Ⅳ,Ⅴ组基础日粮中分别添加10mg/kg,20mg/kg,30mg/kg,40mg/kg的大豆黄酮。试验表明:与Ⅰ组相比,(14日龄)DaⅣ的回肠长度表现差异显著(P<0.05);(28日龄)DaⅣ和DaⅤ的空肠长度表现差异显著(P<0.05);DaⅤ的空肠和回肠的重量均表现差异显著(P<0.05),(42日龄)DaⅡ的十二指肠,空肠,盲肠的长度均表现差异显著(P<0.05),其中回肠的长度表现差异极显著(P<0.01);DaⅤ的空肠重量表现差异显著(P<0.05)。此外(28日龄)DaⅡ的腺胃重量表现差异显著(P<0.05);DaⅤ的鸡体重表现差异显著(P<0.05),其他各组差异不显著。但是整体观察,28日龄和42日龄草杂鸡体重是随添加Da的量的增加而增加。说明大豆黄酮对草杂鸡消化系统的发育有一定的影响。  相似文献   

11.
Bread wheat varieties as influenced by different nitrogen levels   总被引：4，自引：2，他引：2  
INTRODUCTION Dera Ismail Khan lies in Pakistan’s arid zone (71.07° longitude, 31.57° latitude and 500 m above sea level). This zone is in the extreme south of (NWFP) Pakistan at the bank of the River Indus. Dera Ismail Khan is bounded by Sulaiman Range to the West, the Indus River on the East, Marwat and Bhit- tani Ranges on the North and Vehowa Nala (Punjab) on the South. Besides certain local variability the area is comprised of four basic divisions viz mountains, a series …  相似文献   

12.
Stress affects the male reproductive system and can cause sub-fertility or infertility. Although Phyllanthus emblica L. (PE) extract has been shown to have high antioxidant capacity and protective properties in damaged tissue, the preventive effects of PE extract on testicular function from stress-related impairment have never been demonstrated. This study aimed to investigate the effects of PE aqueous leaf extract on testicular impairment and protein marker changes in rats suffering from chronic stress. Adult male rats were divided into four groups: a control group, a chronic stress (CS) group, and two groups with CS that received different doses of PE extract (50 or 100 mg/kg body weight (BW)). In the treatment groups, the animals were given PE extract daily before stress induction for 42 consecutive days. Stress was induced through immobilization (4 h/d) followed by forced cold swimming (15 min/d). Sperm quality and the histology of the testes and caudal epididymis were examined, as were levels of serum corticosterone, testosterone, and malondialdehyde (MDA). The expressions of testicular steroidogenic acute regulatory (StAR) and tyrosine-phosphorylated proteins were investigated using immuno-Western blot analysis, as these proteins are assumed to play important roles in spermatogenesis and androgen synthesis. The results showed that PE (50 mg/kg BW) significantly increased sperm concentration and testosterone levels, while decreasing corticosterone levels, MDA levels, sperm head abnormalities, and acrosome-reacted sperm in CS rats. In addition, PE at both doses was found to diminish testicular histopathology in the CS rats. We also found that 50 mg/kg BW of PE significantly improved StAR protein expression and altered the intensities of some tyrosine-phosphorylated proteins in testis. We conclude that PE leaf extract at 50 mg/kg BW can prevent testicular damage in rats with CS.  相似文献   

13.
低聚壳聚糖对肉仔鸡生长性能及免疫器官发育的影响   总被引：1，自引：0，他引：1  
480羽1日龄AA肉鸡,随机分为4组,每组120羽,设6个重复,其中空白对照组饲喂玉米-豆粕型基础日粮,抗生素组基础日粮添加50mg/kg金霉素,试验Ⅰ组(低聚壳聚糖剂量组)和试验Ⅱ组(黄芪多糖高剂量组)分别添加20mg/kg和40mg/kg低聚壳聚糖,试验期42d,研究低聚壳聚糖对肉仔鸡生长性能及免疫器官发育的影响。结果显示,试验Ⅰ、Ⅱ组肉仔鸡的平均体重、日增重、免疫器官重量及其指数均不同程度高于同期空白对照组和抗生素组,其中平均体重与日增重多差异显著(P<0.05),部分免疫器官重量与指数有统计学意义(P<0.05);平均料重比均较明显地低于同日龄空白对照组和抗生素组。试验结果表明,基础日粮添加20、40mg/kg低聚壳聚糖对肉鸡的生长性能及免疫器官发育具有较明显的促进作用,可提高肉鸡的平均日增重和饲料转化率,增强机体的免疫功能。  相似文献   

14.
INTRODUCTION Transformation, proliferation and migration of vascular smooth muscle cells (VSMCs) into en- domembrane of arteries are the pathological mani- festations of atherosclerosis. Gap junction (GJ) and connexin (Cx) situated between endothelial cells play an important role in this process. Currently, connexin between VSMCs that have been recognized at protein molecular level includes Cx40, Cx43, and Cx37 (Li and Simardm, 1999). It showed that the occurrence of arteriosclerosi…  相似文献   

15.
论文通过文献资料法、逻辑分析法等对骨髓间充质干细胞作用于心肌细胞凋亡应用,以及心肌细胞凋亡与Bcl-2和Bax蛋白表达的关系进行研究,旨在探讨骨髓间充质干细胞调控Bcl-2、Bax基因蛋白表达的方式对心肌细胞凋亡的作用机制。研究发现,心肌细胞凋亡与Bcl-2、Bax基因蛋白的表达存在高度相关性,通过调控Bcl-2、Bax的基因表达能够在一定程度上防止心肌细胞凋亡的发生与发展,而骨髓间充质干细胞则可以通过自身诱导分化的特性有效的调控Bcl-2和Bax蛋白的表达,使损伤后的心肌功能得到改善。  相似文献   

16.
Objective： The prevalence of non-alcoholic fatty liver disease （NAFLD） has markedly increased. Insulin resistance has been implicated in the pathogenesis of NAFLD. This study was aimed at observing the relationship between insulin resistance and NAFLD, and evaluating the role of pioglitazone （PGZ） acting as insulin-sensitizing agents in the prevention and treatment of rat fatty liver induced by high fat feeding. Methods： The rats were separated randomly into 6 groups： model group Ⅰ were fed high fat diet for 8 weeks, PGZ prevention group were given PGZ 4 mg/（kg.d） simultaneously, while control group Ⅰ were fed normal food for 8 weeks; model group Ⅱ were fed high fat diet for 16 weeks, PGZ treatment group were given PGZ 4 mg/（kg.d） orally simultaneous with high fat diet for 8 weeks after high fat feeding for 8 weeks, control group Ⅱ were fed normal food for 16 weeks. The rats were sacrificed after 8 weeks and 16 weeks respectively. Liver weight, body weight, serum activities of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, alkaline phosphatase （ALP）, tumor necrosis factor alpha （TNF-α）, fasting blood glucose （FBG）, fasting plasma insulin （FINS）, HOMA （homeostasis model assessment） insulin resistance index （HOMA-IR）, and the liver histology of rats of all groups were assayed. Results： After 8 weeks, the liver in model group Ⅰ showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration, liver indexes and serum levels of ALT, AST, ALP, TNF-α were significantly increased （P〈0.05） compared with control group Ⅰ. Whereas, the degree of hepatic injury was attenuated in PGZ prevention group, liver indexes and serum levels of ALT, ALP were significantly decreased （P〈0.05） compared with model group Ⅰ. After 16 weeks, notable steatosis, and lobular inflammation were observed in model group Ⅱ rat liver, while the degree of hepatic injury was attenuated in the PGZ treatment group. Liver index, serum levels ofALT, AST, ALP, FINS and HOMA-IR were significantly increased （P〈0.05） in model group Ⅱ compared with control group Ⅱ. Whereas, in PGZ treatment group, serum levels of AST and FINS showed decreasing tendency, liver indexes, serum levels of ALT, ALP, TNF-α and HOMA-IR were significantly decreased compared with model group Ⅱ. Conclusion： Insulin resistance plays a role in the pathogenesis of NAFLD in rats. Pioglitazone can attenuate insulin resistance and biochemical and histological injury in high fat-induced fatty liver in rats.  相似文献   

17.
目的：观察戊四氮（PTZ）致痫大鼠海马神经元caspase-3表达以及中药复方AAP的脑保护作用.方法：144只健康成年雄性Wistar大鼠随机分为对照组（CK组）、模型组（PTZ组）、中药大剂量组（AAPl组）、中药中剂量组（AAPm组）、中药小剂量组（AAPs组）和丙戊酸钠组（VPA组）;每组各6只.CK组和PTZ组分别给予生理盐水（4mL/kg.d）灌胃;中药各组分别给予中药复方大、中、小剂量（10.26g/kg、5.13g/kg、2.56g/kg）灌胃,每天1次;VPA组腹腔注射VPA（20mg/kg.d）.造模第一天,除CK组外,其余各组大鼠均腹腔注射戊四氮（PTZ）75mg/kg,观察记录大鼠行为学变化;于致痫后12h、2d、5d、7d相应时间点取材,制备脑标本;免疫组化检测caspase-3表达.结果：致痫后,除CK组外,其余各组海马区caspase-3阳性表达增强;7天,与PTZ组相比,AAPl组、AAPm组和AAPs组海马CA3区caspase-3阳性表达减弱（P〈0.05）.结论：caspase-3参与致痫大鼠海马神经元凋亡过程;AAP能降低caspase-3表达,减少神经元凋亡,有神经保护作用.  相似文献   

18.
阿尔茨海默病是一种由多种因素引起的神经系统性退化疾病,其表现为β-淀粉样蛋白沉积和Tau蛋白高度磷酸化等。雌激素可以通过抑制天冬氨酸蛋白水解酶（cysteinyl aspartate specific proteinase,caspase）的活性、调节Bcl相关X蛋白（Bcl-associated X protein,Bax）和B淋巴细胞瘤2（B-cell lymphoma 2,Bcl-2）的表达及其比值、提高下丘脑神经元和神经胶质细胞的环磷酸腺苷（cyclic adenosine monophosphate,c AMP）含量、促进c AMP反应元件结合蛋白（c AMP response element-binding protein,CREB）快速磷酸化、影响海马细胞内N-甲基-D-天门冬氨酸（N-methl-D-aspartate,NMDA）受体的表达而抑制神经元的凋亡;雌激素还可通过影响脑源性神经营养因子和突触素,发挥对神经元的直接保护作用。  相似文献   

19.
目的 :通过ENU诱发子代大鼠脑肿瘤的模型 ,对子代鼠脑肿瘤发生前、后p2 1基因的变化进行连续地观察 ,探讨抑癌基因 p2 1与胶质瘤发生、发展的关系。方法 :( 1)通过对孕18d大鼠腹腔注射亚硝基乙基脲 (ENU ,75mg/kg)的方法 ,建立子代鼠脑肿瘤模型 ;( 2 )应用免疫组化的方法 ,对子代鼠 90d、12 0d和 15 0d鼠脑 p2 1蛋白表达进行观察。 结果 :p2 1蛋白的表达减弱或消失 ,见于子代鼠出生后 90d、12 0d和 15 0d ,特别是 90d组 ,且 15 0d组中肿瘤内与瘤周组织之间有统计学差异。结论 :异常 p2 1蛋白的表达存在于肿瘤发生前、发生早期及发生后各阶段 ,进一步说明p2 1WAF1/CIP1发生突变、其表达减弱或消失是脑胶质瘤起源的重要原因。p2 1基因的功能失常可能是导致基因链活动紊乱的因素之一 ,而肿瘤的形成还可能需要其它因素参与。  相似文献   

20.
Paracetamol (PCM) overdose can cause nephrotoxicity with oxidative stress as one of the possible mechanisms mediating the event. In this study, the effects of ethyl acetate extract of Zingiber zerumbet rhizome [200 mg per kg of body weight (mg/kg) and 400 mg/kg] on PCM-induced nephrotoxicity were examined. Rats were divided into five groups containing 10 rats each. The control group received distilled water while other groups were treated with extract alone (400 mg/kg), PCM alone (750 mg/kg), 750 mg/kg PCM+200 mg/kg extract (PCM+ 200-extract), and 750 mg/kg PCM+400 mg/kg extract (PCM+400-extract), respectively, for seven consecutive days. The Z. zerumbet extract was given intraperitoneally concurrent with oral administration of PCM. Treatment with Z. zerumbet extract at doses of 200 and 400 mg/kg prevented the PCM-induced nephrotoxicity and oxidative impairments of the kidney, as evidenced by a significantly reduced (P<0.05) level of plasma creatinine, plasma and renal malondialdehyde (MDA), plasma protein carbonyl, and renal advanced oxidation protein product (AOPP). Furthermore, both doses were also able to induce a significant increment (P<0.05) of plasma and renal levels of glutathione (GSH) and plasma superoxide dismutase (SOD) activity. The nephroprotective effects of Z. zerumbet extract were confirmed by a reduced intensity of renal cellular damage, as evidenced by histological findings. Moreover, Z. zerumbet extract administered at 400 mg/kg was found to show greater protective effects than that at 200 mg/kg. In conclusion, ethyl acetate extract of Z. zerumbet rhizome has a protective role against PCM-induced nephrotoxicity and the process is probably mediated through its antioxidant properties.  相似文献   

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