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目的:探讨硫化氢(H2S)对兔左心室心肌细胞电生理特性的影响.方法:用标准微电极细胞内记录技术,观测硫化氢对兔左心室心肌细胞电生理特性的影响.观测指标:静息电位(resting potential,RP)、超射值(over-shoot,OS)、动作电位幅值(amplitude of action po-tential,APA)、0相最大除极速率(maximal rate of depolarization,Vmax)、复极20%、50%和90%时间(20%、50%and 90% of duration of action potential,APD20、APD50and APD90)及复极化平均速度(average rate of repolarization).结果:(1)用50μmol/L NaHS 灌流时,和正常对照组相比,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).(2)100μmol/L NaHS与对照组比较,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).和50μmol/L NaHS相比APD50缩短(P＜0.01)、APD90缩短(P＜0.05)、复极化平均速度增快(P<0.05).(3)200μmol/L NaHS、400μmol/L、1 000μmol/L NaHS可浓度依赖性的使 APA、RP、OS减小(P<0.01),APD20、APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.01).结论:H2S可浓度依赖性的影响兔左心室心肌细胞电生理特性. 相似文献
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INTRODUCTION In the arsenal of cardiac functional parameters,cardiac output(CO),cardiac index(CI),and strokevolume(SV)are potentially important determinantsof hemodynamics,the measurement of which relieson complicated and sometimes invasive techniques.In the case of congestive heart failure,non-invasivemethods to provide accurate measures of these pa-rameters during exercise stress testing would addsignificant objectiveness to the test result.This couldbe of considerable importance for … 相似文献
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Chen ZW Chen LY Dai HL Chen JH Fang LZ 《Journal of Zhejiang University. Science. B》2008,9(8):616-622
Objective:To investigate the relationship between alanine aminotransferase (ALT)levels and metabolic syndrome (MS)in nonalcoholic fatty liver disease(NAFLD).Methods:A total of 26527 subjects who received medical health checkup in our hospital from January 2005 to July 2007 were enrolled in the study.The diagnosis of fatty liver was based on ultrasound imaging.MS Was defined according to the criteria of the Adult Treatment Panel Ⅲ.ALT,triglyceride(TG),high density lipoprotein cholesterol(HDL-c),fasting plasma glucose(FPG),height,weight,waist circumference(WC),systolic blood pressure (SBP)and diastolic blood pressure(DBP)were measured in each subject to analyze the relationship between MS and ALT activity.Results:(1)The prevalence of NAFLD in men(30.94%)was significantly higher than that in women(15.65%);(2)The incidence of MS in NAFLD(33.83%)was significantly greater than that in non-NAFLD(10.62%);(3)Of the 6470 subjects with NAFLD,in the age-adjusted partial correlation analysis,there were statistically significant correlations between the ALT levels and most metabolic risk factors in each sex(P＜0.01),except that ALT levels had no correlation with HDL-c in women.Moreover,in the multiple stepwise regression analysis,SBP lost its significance,and WC,body mass index(BMI),age,DBP,TG and FPG were independently associated with ALT levels in both sexes (P＜0.05).HDL-c remained significant and was independently related to ALT leveis in men;(4)ALT levels were significantly higher in subjects with MS compared to those without MS(P＜0.001).Mean ALT levels increased with the number of MS cornponents in each sex (P.＜0.05 for trend).Conelusion:We found a strong relationship between ALT leveIs and MS in NAFLD and revealed that the cluster of MS components might be the predictor for ALT elevations. 相似文献
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Hong-xia Che Bo Tian Li-na Bai Li-ming Cheng Li-li Liu Xiao-na Zhang Zhan-mei Jiang Xiao-xi Xu 《Journal of Zhejiang University. Science. B》2015,16(8):672-679
Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3'',5,5''-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1–2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk. 相似文献
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Effect of enalapril on plasma homocysteine levels in patients with essential hypertension 总被引：1，自引：0，他引：1
Fang-fang Fan Yong Huo Xu Wang Xin Xu Bin-yan Wang Xi-ping Xu Jian-ping Li 《Journal of Zhejiang University. Science. B》2010,11(8):583-591
Objective:To investigate the effect of enalapril on plasma homocysteine(Hcy) levels and the association of methylenetetrahydrofolate reductase(MTHFR) C677T polymorphism with the changes of Hcy levels in response to enalapril among patients with essential hypertension.Methods:A total of 130 patients with mild-to-moderate essential hypertension were enrolled and enalapril was orally administered at a dose of 10 mg/d for eight weeks.Plasma Hcy levels were measured by denaturing high-performance liquid chromatography(DHPLC) at baseline and after eight weeks of treatment.Genotyping of MTHFR C677T polymorphism was performed by TaqMan probe technique.Results:Compared with baseline,plasma Hcy levels did not change significantly after eight weeks(P=0.81).Stratified by baseline Hcy levels,a significant increase in plasma Hcy levels(P=0.02) among those with Hcy 10 μmol/L was observed,in contrast to no significant changes in plasma Hcy levels(P=0.54) among those with Hcy ≥10 μmol/L.No significant association was observed between MTHFR C677T polymorphism and changes in Hcy levels in response to enalapril.Conclusions:Enalapril may cause an increase in plasma Hcy levels among the hypertensives with low baseline Hcy levels.There was no significant association between MTHFR C677T genotypes and changes in Hcy levels in response to enalapril among subjects with essential hypertension. 相似文献
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Objective: We aim to describe the environment iodine concentration in salt, water and soil along Zhejiang Province coast in the China foreland. It will be helpful for us to judge whether this area is insufficient in iodine and universal iodized salt is necessary or not. Methods: We collected iodized salt samples, drinking water samples (tap water in the towns, and well water or spring water in the villages), water samples from different sources (ditches, lakes, rivers) and soil samples through random sampling in June, 2005. Salt, water and soil iodine was detected by arsenic-cerium redox method. Statistical analysis was expressed as mean±SEMby Windows SPSS 13.0. Results: (1) The iodine concentration in salt was 27.9±4.33 mg/kg (n=108). (2) Seventy-five water samples were collected. The water iodine value was 0.6～84.8 μg/L (mean of 11.66 μg/L). The watershed along the Qiantang River has significantly higher iodine content than the water in Lin'an in mountain area (P＜0.01). The iodine content and mean iodine content of tap water, well or spring water and natural water sources were 4.30±2.43 μg/L (n=34), 23.59±27.74 μg/L (n=19)and 12.72±10.72 μg/L (n=22) respectively. This indicated that among environmental water sources, the ditch iodine content was the highest with river water iodine being the lowest (P＜0.01). (3) Soil iodine value was 0.11～2.93 mg/kg (mean of 1.32 mg/kg).Though there was no statistical difference of soil iodine in different districts (P=0.131), soil iodine content correlated positively with water iodine content. Conclusion: Iodine concentration in salt accords with national policy of adding iodine in salt. Foreland has more iodine in water than mountain area. The data reflected that water and soil iodine in foreland area was not high, which suggests universal iodized salt should be necessary. Environment iodine has relatively close association with pollution. 相似文献
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Comparison of an SMS text messaging and phone reminder to improve attendance at a health promotion center： A randomized controlled trial 总被引：2，自引：0，他引：2
Objective To compare the efficacy of a short messaging service (SMS) text messaging and phone reminder to improve attendance rates at a health promotion center. Methods A total of 1 859 participants who had scheduled appointments in the health promotion center of our hospital from April 2007 to May 2007 were enrolled in the study and randomly assigned into 3 groups control (no reminder) group, SMS text messaging reminder group and telephone reminder group. Attendance rates and costs of interventions were collected. Results A total of 1848 participants were eligible for analysis. Attendance rates of control,SMS and telephone groups were 80.5%, 87.5% and 88.3%, respectively. The attendance rates were significantly higher in SMS and telephone groups than that in the control group, with odds ratio 1.698, 95% confidence interval 1.224 to 2.316, P=0.001 in the SMS group, and odds ratio 1.829, 95% confidence interval 1.333 to 2.509, P＜0.001 in the telephone group. However, there was no difference between the SMS group and the telephone group (P=0.670). The cost effectiveness analysis showed that the cost per attendance for the SMS group (0.31 Yuan) was significantly lower than that for the telephone group (0.48 Yuan). ConclusionSMS and telephone are effective reminders for improving attendance rate at a health promotion center. SMS reminder may be more cost-effective compared with the telephone reminder. 相似文献
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INTRODUCTION Acute pulmonary thromboembolism(PTE)is serious pulmonary vascular disease with high mortality.The challenge is to make a correct diagnosiand give appropriate treatment(Nicolaides et al.2001).Effective treatment will decrease morbidityand mortality.This study compares the efficacy,adverse effects and costs of low-molecular-weightheparin(LMWH)and unfractionated heparin(UH).MATERIALS AND METHODS Materialss a or-sis al.,ity ad-ht-).All patients diagnosed as non-massiv… 相似文献
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INTRODUCTION Colorectal cancer is the most common cancer ofthe Western world and accounts for approx.Elevenpercent of all cancer deaths in the United States.Aswith other malignancies,colorectal adenocarcinomais thought to develop through the accumulation ofgenetic alterations that inhibit cell growth.Two of theoncogenes that have been implicated in this processare bcl-2and p53,the products of which are involvedin apoptosis,cell proliferation and tumor develop-ment(Garewal et al.,1996).S… 相似文献
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Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer ) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15),peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases. 相似文献
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GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo／reperfusion rat 总被引：3，自引：0，他引：3
Liu JR Ding MP Wei EQ Luo JH Song Y Huang JZ Ge QF Hu H Zhu LJ 《Journal of Zhejiang University. Science. B》2005,6(4):254-258
INTRODUCTION GM1 ganglioside (GM1) is the main kind ofgangliosides in mammalia, and most abundant inbrain tissue (Duchemin et al., 2002). It was reportedthat GM1 could protect cerebral ischemia in vivo andin vitro, one protective mechanism of which is thatGM1 could reduce neural injury induced by toxicityof excitatory amino acid via N-methyl-D-aspartate receptor (NMDAR) (Kharlamov et al., 1993; Simon et al., 1993; Garofalo and Cue… 相似文献
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DíAZ Marlén Pérez RIZO Oscar Díaz DíAZ Adlin López APARICIO Eric Estévez DíAZ Reinaldo Roque 《Journal of Zhejiang University. Science. B》2006,(12)
INTRODUCTION Image quality in nuclear medicine tomography is critically dependent on the activity undergone by the patient. The optimal activity is the smallest amount of activity which preserves diagnostic accuracy. Nev- ertheless, the optimum depends on the gamma camera used for imaging, the size of the patient and the im- aging application (Mattsson et al., 1998). To optimize, one has to study how diagnostic accuracy depends on the activity undergone for each particular study, taking i… 相似文献
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This paper focused on the removal and transformation of the dissolved organic matter （DOM） in secondary effluent during the granular activated carbon （GAC） treatment. Using XAD-8/XAD-4 resins, DOM was fractionated into five classes： hydrophobic acid （HPO-A）, hydrophobic neutral （HPO-N）, transphilic acid （TPI-A）, transphilic neutral （TPI-N） and hydrophilic fraction （HPI）. Subsequently, the water quality parameters of dissolved organic carbon （DOC）, absorbance of ultraviolet light at 254 nm （UV-254）, specific ultraviolet light absorbance （SUVA） and trihalomethane formation potential （THMFP） were analyzed for the unfractionated and fractionated water samples. The results showed that the order of the DOC removal with respect to DOM fractions was observed to be HPI〉HPO-A〉HPO-N〉TPI-A〉TPI-N. During the GAC treatment, the THMFP of the unfractionated water samples decreased from 397.4 μg/L to 176.5 μg/L, resulting in a removal efficiency of 55.6%. The removal order of the trilaalomethanes （THMs） precursor was as follows： HPO-A〉TPI-A〉TPI-N〉HPO-N〉HPI. By the GAC treatment, the specific THMFP of HPO-A, TPI-A, TPI-N and the original unfractionated water samples had a noticeable decrease, while that of HPO-N and HPI showed a converse trend. The Fourier transform infrared （FTIR） results showed that the hydroxide groups, carboxylic acids, aliphatic C-H were significantly reduced by GAC treatment. 相似文献
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We examined the effects of conjugated linoleic acid (CLA) on growth, fatty acid composition and enzyme activity of fatty acid oxidation in the liver of large yellow croaker. We divided 1600 fish (average initial weight 150 g) into 4 groups and reared them in 8 cages. Four dietary treatments were formulated to contain 0%, 1%, 2% and 4% (w/w) CLA, respectively. The fish were fed for 10 weeks ad libitum twice daily. We found that the dietary CLA had no effect on growth, biometric parameters and whole body proximate (P>0.05), but showed some significant effects on the fatty acid composition in both muscle and the liver.The activities oflipogenic enzymes were slightly depressed in fish fed with increasing levels of CLA when compared with control (P>0.05). Dietary CLA supplementation had no effects on liver lipid content, but significantly increased the contents of saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) (P＜0.05) and decreased monounsaturated fatty acid (MUFA) content in both muscle and the liver. Dietary CLA inclusion resulted in significant increases of the biologically active cis-9, trans-11 and trans-10, cis-12 isomers in both tissues (P＜0.05). The total accumulation of CLA was higher in the liver (3.83%, w/w) than in muscle (3.77%, w/w) when fed with 4% (w/w) CLA. This study demonstrates that large yellow croakers are capable of absorbing and depositing CLA and long-chain n-3 PUFA in the liver and muscle, showing that this species fed with CLA could be an important human food source for these healthful fatty acids. 相似文献
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利用方波伏安法研究了尿酸在聚甘氨酸修饰玻碳电极上的电化学氧化行为.结果表明:在0.01 mol/L磷酸盐缓冲液(pH=7)中,尿酸在+0.31 mv左右产生一个氧化峰,峰电流与尿酸在1.0×10-6—1.0×10-5mol/L浓度范围呈较好的线性关系,相关系数为0.9945.该方法可用于直接测定人体尿酸,回收率为95.5%-104.1%,相对标准偏差为1.4%. 相似文献
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目的:探讨胸苷酸合成酶(TS)和雌激素受体(ER)在乳腺癌中的表达及它们的相关性.方法:采用免疫组织化学SP法检测111例乳腺癌和38例乳腺腺瘤组织中TS和ER的表达情况.结果:乳腺腺瘤的TS和ER阳性率分别为10.53%(4/38)和36.84%(14/38),差异有显著性(P<0.05);乳腺癌病人中TS和ER的阳性率分别为31.53%(35/111)和40.54%(45/111),差异无显著性(P>0.05).乳腺癌中TS和ER的表达水平与患者年龄、发生部位、组织学类型、淋巴结转移相关性不明显(P>0.05).TS和ER在乳腺腺瘤组呈正向相关(r=0.077),而在乳腺癌组呈负向相关(r=-0.016).结论:TS在乳腺癌组表达率明显高于腺瘤(P<0.0 5);ER在良、恶性乳腺肿瘤内表达率差异无显著性(P>0.05).检测TS、ER对化疗药物选择有指导意义. 相似文献
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INTRODUCTION In 1998 gastrointestinal pathologists reached aconsensus on the definition of chronic atrophic gas- tritis (CAG), which was described as programmed loss of gastric gland and/or replacement by intestinal glands in gastric mucosa. CAG was recognized to be closely related with development of gastric cancer and listed as precancerous lesions in this meeting (Genta, 1998). According to Correa (1992)’s cascade of gas-tric carcinogenesis, gastric cancer was believed to develop fr… 相似文献
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研究了钛铁试剂分光光度法测定土壤中铁的方法,探讨了方法的最佳条件。结果表明,以钛铁试剂为显色剂,可于560nm处测定土壤中铁的含量,铁含量在0-3mg／L范围内服从比尔定律,线性回归方程为Y=0．0889x,相关系数1=0．9984,表观摩尔吸光系数为K560=5．12×10^4L·mol^-1·cm^-1。该方法简便,选择性好,灵敏度高。此法用于土壤中铁的测定,结果与磺基水杨酸法相符。样品标准加入回收率分别为95．6％～99．9％,相对标准偏差为0．34％-0．53％（n=6）。 相似文献
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利用初等微分学比较了单参数平均与对数和指数平均的几何组合,发现了使得双向不等式Jp（a,b）1/2-3)/2]和所有a,b>0且a≠b成立的p的最大值和q的最小值,其中Jp（a,b）,L（a,b）和I（a,b）分别表示a与b的p-次单参数平均、对数平均和指数平均. 相似文献