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Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity 总被引：1，自引：0，他引：1
INTRODUCTIONInterleukin-18(IL-18),originallydescribedasinterferon(IFN)-g-inducingfactor,isan18.3-kDaproinflammatorycytokineproducedbyactivatedmacrophagesanddendriticcells,andplaysanim-portantroleintheTh1response,primarilybasedonitsabilitytoinduceIFN-gproductioninTcellsandNKcells.IL-18inducesproliferationofactivatedTcells,secretionofseveralcytokines,andpartici-patesinbothinnateandacquiredimmunity.TheroleofIL-18inantitumorimmunitywassuggestedinmanystudies.Genetransferofcytokinesisani… 相似文献
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Inducible nitric oxide synthase expression is related to angiogenesis, bcl-2 and cell proliferation in hepatocellular carcinoma 总被引：3，自引：0，他引：3
ＩＮＴＲＯＤＵＣＴＩＯＮＮｉｔｒｉｃｏｘｉｄｅ (ＮＯ) ,ａｓｈｏｒｔｈａｌｆ ｌｉｆｅｒａｄｉｃａｌ,ｉｓｈｉｇｈｌｙｒｅａｃｔｉｖｅ ,ａｎｄｉｓｉｎｖｏｌｖｅｄｉｎｍａｎｙｂｉｏ ｌｏｇｉｃａｌｐｒｏｃｅｓｓｅｓ,Ｉｔｓｅｅｍｓｔｏｐｌａｙａｎｉｍｐｏｒｔａ 相似文献
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INTRODUCTION Biochip is developed since the 1990s high-throughput technology tool developed for mo- lecular biology research in the biological technology revolution (Watson and Akil, 1999) that closely fol- lowed the advent of Large Scale Integrated circuit. Biochip is usually divided into several different kinds, such as gene chip, protein chip, cell chip, microbiol- ogy chip, tissue chip and so on. In 1998, Kononen et al.(1998) reported the technology of tissue chip: tis- sue microarr… 相似文献
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In this study, we examined the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor
(VEGF) by immunohistochemical staining in 76 tissue sections collected from hepatocellular carcinoma (HCC) patients undergoing
hepatectomy. Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody.
We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC. Most
of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody. The iNOS stain intensity of the liver tissue
close to the tumor edge was stronger than that of HCC tissue, and the strongest was the hepatocytes closer to the tumor tissue.
However, iNOS expression in 10 normal hepatic samples was undetectable. VEGF positive expression ratio was 84.8% in iNOS positive
expression cases, and the ratio was 35.3% in negative cases. There was significant correlation (P=0.000) between iNOS and VEGF expression. Moreover, iNOS expression was significantly associated with bcl-2 and MVD, but without
p53 expression. DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the
cell cycle in HCC. PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF
group was significantly higher than that in the combined negative group. The present findings suggested that iNOS expression
was significantly associated with angiogenesis, bcl-2 and cell proliferation of HCC.
Project supported in part by the National Ninth-Five-Years Project Fund (No. 96909121), China 相似文献
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To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhIL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hIL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3±4.5 pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine. 相似文献
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INTRODUCTION Colorectal cancer is the most common cancer ofthe Western world and accounts for approx.Elevenpercent of all cancer deaths in the United States.Aswith other malignancies,colorectal adenocarcinomais thought to develop through the accumulation ofgenetic alterations that inhibit cell growth.Two of theoncogenes that have been implicated in this processare bcl-2and p53,the products of which are involvedin apoptosis,cell proliferation and tumor develop-ment(Garewal et al.,1996).S… 相似文献
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