共查询到20条相似文献,搜索用时 62 毫秒
1.
J Kato Alice Abraham Ruram S Sekharjit Singh S Bilasini Devi Th Ibetombi Devi W Gyaneshwar Singh 《Indian journal of clinical biochemistry : IJCB》2007,22(1):128-130
The present, study was conducted to determine the level of malondialdehyde (MDA) as an index of free radial induced lipid
peroxidation and antioxidant vitamins-vitamins A, vitamin C and vitamin E in 75 confirmed cases of urolithiasis. Significantly
high level of MDA (p<0.001) with significantly low levels of vitamin E (p<0.001) and vitamin A (p<0.001) with no significant
decrease in vitamin C (p>0.05) were observed in the plasma of urolithiasis cases as compared to normal controls. In conclusion,
it appears that a role of lipid peroxidation and oxidative function exists in the pathogenesis of urolithiasis. But, the exact
mechanism how this occurs remains to be elucidated. 相似文献
2.
Kiran Dahiya A. D. Tiwari Vijay Shankar Simmi Kharb Rakesh Dhankhar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):157-160
Phototherapy has been related to increased oxidative stress and lipid peroxidation. In the present study, thirty full term
jaundiced neonates with appropriate weight were analyzed before and after completion of phototherapy for malondialdehyde (MDA),
reduced glutathione (GSH), total thiols, vitamin C and superoxide dismutase (SOD) levels in hemolysate and albumin levels
in plasma. These parameters were analyzed in cord blood samples of 20 healthy neonates as control. It was observed that levels
of MDA were elevated significantly (p<0.001) in patients as compared to controls and that the levels increased significantly
after phototherapy (p<0.001). Levels of SOD were also found to be increased significantly as compared to controls and the
levels rose after phototherapy (p<0.001). On the other hand, the levels of non-enzymatic antioxidants such as GSH, total thiols
and vitamin C were significantly low (p<0.001) as compared to controls and the levels decreased significantly after phototherapy
(p<0.001). The plasma albumin levels also were found to be decreased significantly after phototherapy (p<0.01). Therefore,
phototherapy increases oxidative stress and should be used with care. 相似文献
3.
Vasavidevi V. Bet Kishor H. Deshpande Adinath N. Suryakar Rajesh D. Ankush Raghavendra V. Katkam 《Indian journal of clinical biochemistry : IJCB》2006,21(2):177-180
Crystal aggregation and retention are critical events for the formation of kidney stones. There is a close association between
crystal development and free radical activity in vivo. In the present study 30 subjects presenting with urolithiasis were
included. Serum levels of total lipid peroxides, nitric oxide (as nitrite), α-tocopherol, plasma ascorbic acid (vitamin C)
and erythrocyte superoxide dismutase activity were measured. These findings were compared with 30 age matched control subjects
irrespective of sex. Student's ‘t’ test was applied for statistical analysis. There was a significant increase in lipid peroxides
(p<0.001), where as significant decrease in nitrite (p<0.01) and α-tocopherol (p<0.001) levels were observed. Plasma ascorbate
(p>0.05) and erythrocyte superoxide dismutase activity (p>0.05) was also found to be decreased but the difference was not
statistically significant which suggests that oxidative stress is evident in urolithiasis with depletion in antioxidant status
where as decrease in nitric oxide may be less abetting in disease condition. 相似文献
4.
S. Mohanty P. K. Sahu M. K. Mandal P. C. Mohapatra A. Panda 《Indian journal of clinical biochemistry : IJCB》2006,21(1):101-105
This work was undertaken to investigate correlation between oxidative stress and initiation of pathogenesis of pregnancy induced
hypertension (PIH). Fifty primigravidae in age group of 20–35 years and gestational age 28–42 weeks with PIH were taken as
cases. Twenty healthy primigravidae with no medical and surgical complications of pregnancy and with blood pressure ≤140/90
mm Hg served as controls. The cases were again subgrouped as severe preeclampsia (12 in number) and mild pre-eclampsia (38
in number). All of them were evaluated for serum malondialdehyde (MDA), Serum vitamin E and plasma vitamin C levels. The serum
MDA levels were raised significantly in women with mild preeclampsia (P<0.01) and in women with severe preeclampsia (P<0.01)
in comparison to normal primi gravida. The serum vitamin E levels were decreased in primi gravida with mild preeclampsia (p<0.1)
and in primi with severe pre eclampsia (P<0.1) in comparison to normal primi gravida but the fall was not statistically significant.
There was a significant fall (P<0.05) in the vitamin C levels in primi with mild preeclampsia than in the normal primi. The
vitamin C levels in severe preeclamptic patients were lower than the normal primi but the fall was not statistically significant
(P=0.10). The serum MDA and vitamin E showed a negative correlation in all the cases. The serum MDA and plasma vitamin C also
showed a negative correlation in the control and study group. This observation suggests that in hypertensive disorders of
pregnancy there is an imbalance between lipid peroxidation and antioxidant vitamin status because of oxidative stress. The
decreased serum concentrations of the antioxidant vitamins supports the hypothesis that lipid peroxidation is an important
causative factor in the pathogenesis of preeclampsia. The rise in antioxidants is probably to compensate the increased peroxide
load in severe preeclampsia. 相似文献
5.
Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR) 总被引:1,自引:0,他引:1
Ullas Kamath Guruprasad Rao Shobha U Kamath Lavanya Rai 《Indian journal of clinical biochemistry : IJCB》2006,21(1):111-115
The present study demonstrates the possibility of increased lipid peroxidation and protein oxidation in both maternal and
fetal erythrocytes as markers of oxygen radical activity during intrauterine growth retardation. The erythrocyte MDA levels
were significantly elevated in mothers of IUGR babies when compared to controls (p<0.01). The endogenous protein damage due
to oxidative stress was significantly higher in IUGR mothers when compared to controls (p<0.05). Similarly the proteolytic
activity in erythrocyte lysates against oxidatively damaged hemoglobin was significantly increased in mothers of IUGR babies
compared to controls (p<0.001).
In fetuses born with IUGR, both lipid peroxidation and proteolytic activity were significantly increased when compared to
normal newborns (p<0.01).
The result of this study indicates that oxidative stress was induced both in IUGR babies and their mothers which is manifested
as increased lipid peroxidation and protein oxidant damage. 相似文献
6.
The objective of the present study is to evaluate the beneficial effect of tomatoes, which are rich source of Lycopene, relatively
a new carotenoid known to play an important role in human health and disease. In this study lipid peroxidation rate was measured
by estimating Malondialdehyde and the levels of serum enzymes involved in antioxidant activities like Super Oxide Dismutase,
Glutathione Peroxidase, Glutathione Reductase, Reduced Glutathione, in type-II diabetic group (n=40) and age matched control
group (n=50), and observed significantly lower levels of antioxidant enzymes and very high lipid peroxidation rate in type-II
diabetes when compared to control group (p<0.001). Short term supplementation with tomatoes (cooked) to diabetic group for
a period of 30 days, showed a significant improvement in antioxidant enzyme levels (p<0.001) and decreased lipid peroxidation
rate (p<0.001) suggesting the supplementation with tomato lycopene may serve as the best method of preventing the oxidative
stress in diabetic patients. 相似文献
7.
Serge Herve Tiyong Ifoue C. Teugwa Mofor I. Gouado G. Teto T. Asonganyi P. H. Amvam Zollo 《Indian journal of clinical biochemistry : IJCB》2009,24(3):288-293
Oxidative stress is thought to be involved in the pathophysiology of malaria, especially in pregnancy where natural resistance
is markedly reduced. In the present study we investigated oxidative stress in 315 pregnant women out of which 159 had Plasmodium
falciparum malaria and 154 controls. We evaluated the level of lipid peroxidation products (MDA level) in the plasma, the
activity of erythrocyte antioxidant defense enzymes, superoxide dismutase (SOD, EC: 1.15.1.1) and catalase (Cat, EC: 1.11.1.6)
as well as the ability to resist oxidative stress by the FRAP (Ferric Reducing Ability of Plasma) assay. Total erythrocyte
protein levels were also examined. For the two groups of patients, several differences between the biochemical parameters
tested were found. Median parasitaemia in women with malaria was 25,392 parasites/μl of blood (Range1200–82000), while in
controls we had no parasites found in thin and thick smears. Levels of lipid peroxidation products (MDA) were significantly
higher in patients with parasitemia than in healthy asymptomatic volunteers (mean: 0.844 ± 0.290 and 0.384 ± 0.129 respectively,
p<0.001). This MDA level was higher in primigravidea and also correlates well with parasite density (p<0.001). Catalase activity
in erythrocytes of women with malaria did not differ statistically from that of controls. In contrast, SOD activity of patients
with malaria was found to be significantly higher than that of controls (mean: 0.7899 ± 0.2777 and 0.4263 ± 0.2629 respectively,
p<0.05). FRAP values declined, from parasitemic patients (1.4619 ± 0.6565) compare to controls (2.4396 ± 0.8883, p<0.05),
particularly in the first and third trimester of gestation (p<0.05 and p<0.01 respectively). Finally, total erythrocyte protein
concentrations of women with malaria did not differ from that of the controls. Our results suggest an imbalance between oxidants
and antioxidants in pregnant women suffering from malaria, a situation which could lead to severe damage for either the mother
or the fetus. Therefore, further research should be done to assess the potential benefits of antioxidant supplementation for
the pregnant women suffering from malaria. 相似文献
8.
Guruprasad Rao Ullas Kamath Chaerkadi Raghothama K. Sujatha Pradeep Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2003,18(2):80-86
The present study demonstrates the incidence of increased lipid peroxidation and protein oxidation in both maternal and fetal
erythrocytes as markers of oxygen radical activity in different complications of pregnancy. In fetuses born after premature
rupture of membranes, lipid peroxidation was significantly elevated as indicated by increased malondialdehyde levels (p<0.05)
as compared to controls. Proteolytic activity in the erythrocytes of mothers in this group was also significantly high (p<0.01).
In patients delivered by lower segment cesarian section, lipid peroxidation and proteolytic activity in maternal erythrocytes
were significantly high (p<0.05 and p<0.001 respectively). In patients with prolonged second stage of labour, lipid peroxidation
and proteolytic activity in maternal erythrocytes was significantly higher than in controls (p<0.001 and p<0.05 respectively).
In this group, endogenous protein damage due to oxidative stress was significantly high both in the mother and the fetus (p<0.001
and p<0.05 respectively). 相似文献
9.
S. D. Walwadkar A. N. Suryakar R. V. Katkam K. M. Kumbar R. D. Ankush 《Indian journal of clinical biochemistry : IJCB》2006,21(2):134-137
Generation of reactive oxygen species is an important factor in the development and maintenance of rheumatoid arthritis (RA)
in humans. This study was undertaken to investigate interplay among oxidants, antioxidants and pathogenesis of Rheumatoid
arthritis. Serum levels of lipid peroxides, nitric oxide, vitamin E and ratio of calcium/phosphorus in RA patients were determined
and compared with normal healthy controls. Significant increases in lipid peroxides (p<0.001) and nitric oxide (p<0.001) levels
were found in patients presenting with RA as compared to controls. Whereas significant decrease in vitamin E (P<0.001) and
calcium/phosphorus ratio (p<0.001) were found in Rheumatoid arthritis patients as compared to controls. Positive correlation
was found between lipid peroxides and nitric oxide as well as between vitamin E and calcium. While lipid peroxides and nitric
oxide were correlated negatively with vitamin E. whereas negative correlation was observed between MDA and Calcium/Phosphorus
ratio in patients with rheumatoid arthritis. Our findings suggest that there is a close association between bone loss and
oxidative threat in patients presenting with Rheumatoid arthritis. 相似文献
10.
Nosratollah Zarghami Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):86-91
It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare
seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2α), malondialdehyde (MDA), and total (sum of free and bound) homocysteine
(tHcy) from normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation
products. The study was a case-control study with a simple random sampling. The case group was consisted of 15 asthenozoospermic
males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2α-isoprostane and tHcy were measured
using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay.
The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation
analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. MDA levels
were higher in asthenozoospermic subjects than in control subjects (0.72±0.06 μM vs. 0.40±0.06 μM; p<0.05). No differences
were seen in 15-F2α-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml;
p>0.05). Interestingly, tHcy levels were to be slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 μM
vs. 4.8±0.52 μM). Sperm motility was inversely correlated with seminal plasma 15-F2α-isoprostane and MDA levels, respectively
(p<0.05). In summary, seminal plasma levels of 15-F2α-isoprostane and tHcy showed no significant difference between normozoospermic
and asthenozoospermic men. Sperm motility was not correlated with seminal plasma levels of tHcy. No relationship was found
between tHcy and lipid peroxidation. 相似文献
11.
Sarita N. Chavan Umesh More Shruti Mulgund Vishal Saxena Alka N. Sontakke 《Indian journal of clinical biochemistry : IJCB》2007,22(2):101-105
Osteoporosis encompasses a wide spectrum of conditions associated with imbalance of osteoclastic and osteoblastic activities.
The increased activity of osteoclasts leads to increased free radical formation and hence lipid peroxidation. Present study
probes into the role of antioxidants as a palliative treatment for osteoporosis. It involved 50 healthy controls and 75 clinically
diagnosed osteoporosis patients. Both the groups underwent baseline assessment of biochemical markers viz. osteoblastic markers:
serum Alkaline phosphatase. Free or ionic calcium and Inorganic phosphorus, osteoclastic markers: serum Tartarate resistant
acid phosphatase and Malondialdehyde and the antioxidant status: serum Superoxide dismutase and Erythrocyte reduced glutathione.
The osteoporotic group was then divided into groups A (Vitamin E-Evinal 400 mg), B (Vitamin C-Celin 500 mg), C (Vitamin E+C-Evinal+Celin)
for antioxidant supplementation for a period of 90 days. The results reveal that there is significant fall in concentration
of serum MDA (p<0.001), TrACP (p<0.01). Improvement in antioxidant status is reflected by significant rise in concentration
of serum SOD (p<0.001) and erythrocyte GSH (p<0.001) after 90 days of antioxidant supplementation in osteoporosis. The findings
indicate that on the whole bone status improved with prolonged antioxidant vitamin supplementation, which can be used as a
palliative treatment for osteoporosis. The efficacy is not affected whether the vitamins are administered singly or conjointly. 相似文献
12.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
13.
Bhaskar S Veena Sharmila Upadhya Satish Kumar Adiga Kumar N Pratap 《Indian journal of clinical biochemistry : IJCB》2008,23(2):186-190
The objective of the study was to estimate the serum concentrations of oxidative stress markers-lipid hydroperoxide (MDA-Malondialdehyde),
lactate dehydrogenase (LDH), nitrite; and ferric reducing antioxidant power (FRAP) — a measure of total antioxidant capacity
in serum. Serum nitrite levels were high in fertile women than in infertile women (p<0.001), whereas MDA was high in infertile
women than in fertile group (p<0.018). On classifying the entire group of women with infertility based on the etiology, serum
MDA and LDH levels were higher in women with infertility due to unexplained factor than in women with other causes contributing
to infertility compared to controls (p<0.001, p<0.02). A positive correlation among serum prolactin and nitrite suggested
that hyperprolactinemia could contribute to infertility by inducing oxidative damage. Serum FRAP levels showed no significant
difference in the groups studied. Lipid peroxidation was high in women with unexplained infertility which may be due to hyperprolactinemia. 相似文献
14.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
15.
Arun Kumar Ramiah Sivakanesan Susil Gunasekera 《Indian journal of clinical biochemistry : IJCB》2008,23(3):296-298
The objective of the study was to observe the correlation between oxidative stress and antioxidants in myocardial infarct
patients with normal lipid profile. In the present study investigation of lipid peroxidation and antioxidants were done in
patients. This study was carried out on 165 AMI patients, (123 males and 42 females). The control group consisted on 165 normal
healthy age-sex matched subjects (123 males and 42 females). Levels of Superoxide dismutase, Glutathione Peroxidase, Catalase,
Malondialdehyde and Conjugated dienes were measured. The study found Superoxide dismutase, Glutathione Peroxidase, Catalase
were significantly lower in activities (p<0.001) in AMI patients compared to controls. The markers of lipid peroxidation namely
Malondialdehyde and Conjugated dienes were higher in AMI patients as compared to controls (p<0.001). The present study is
clearly suggestive of increased oxidative stress in AMI patients. Oxidative stress appears as an etiological factor for myocardial
infraction as a consequence the free radical scavengers levels are lowered in AMI patient. 相似文献
16.
Soma Gupta M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2004,19(1):138-141
Lipid peroxidation product, malonaldehyde (MDA) and antioxidants were estimated in plasma and erythrocytes of 34 cases of
oral submucous fibrosis (OSMF) of different grades with equal number of healthy controls to evaluate the association of reactive
oxygen species (ROS) and OSMF. While plasma MDA was found to be significantly higher in patients (3.3±0.4 nmole/ml, P<0.001)
as compared to controls (2.4±0.5 nmole/ml), plasma beta carotene and vitamin E levels were found to be decreased significantly
in patients (81.7±14.3 μg/100 ml, P<0.001; 9.3±0.9 mg/L, P<0.01 respectively) with respect to healthy controls (110±20.8 μg/100
ml and 10.1±1.2 mg/L). The decrease in beta-carotene and vitamin E was found to be more significant in OSMF grade II and III
than in grade I. After 6 weeks of oral administration of beta-carotene and vitamin E, patients showed increase in plasma level
of these two antioxidants along with decrease in MDA level associated with clinical improvement. 相似文献
17.
Eshrat M. Halim A. K. Mukhopadhyay 《Indian journal of clinical biochemistry : IJCB》2006,21(2):181-188
This study was carried out to see the effect of the aqueous extract ofOcitum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male
rats. Adult albino male rats weighing 150–200 gm were made diabetic by intraperitoneal injection of streptozotocin in the
dose 60 mg/kg in citrate buffer (pH 6.3). The diabetic animals were left for one month to develop retinopathy. Biochemical
parameters like plasma glucose, oral glucose tolerance and glycosylated hemoglobin HbA1c, were measured along with lipid profile, and enzymes like glutathione peroxidase (GPX), lipid peroxidase (LPO), superoxide
dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) in normal, untreated diabetic rats and diabetic rats treated
withOcimum sanctum L extracts and vitamin E. Fluorescein angiography test was done for assessing retinopathy. Results on biochemical parameters
were analyzed statistically by using ANOVA followed by Dunnet's ‘t’-test. A p-value of <0.05 was considered as significant.
Evaluation of biochemical profile in treated groups showed statistically significant reduction in plasma levels of glucose,
HbA1c, lipid profile and LPO, and elevation of GPX, SOD, CAT and GST. Treatment of the diabetic animals withOcimum sanctum and Vitamin E, alone and in combination for 16 weeks showed reversal of most of the parameters studied including plasma glucose
levels. Angiography showed improvement in retinal changes following combined antidiabetic treatment. 相似文献
18.
Rahul A. Ghone K. M. Kumbar A. N. Suryakar R. V. Katkam N. G. Joshi 《Indian journal of clinical biochemistry : IJCB》2008,23(4):337-340
Repeated blood transfusion in beta thalassemia major patients may lead to peroxidative tissue injury by secondary iron overload.
In the present study, 72 children with beta thalassemia major were included. Serum levels of total lipid peroxides, Iron,
Total Iron Binding Capacity, Copper, Zinc, Vitamin E, plasma Total Antioxidant Capacity, activity of Erythrocyte Superoxide
Dismutase, were measured. The findings were compared with 72 age matched healthy controls irrespective of sex. A significant
increase in the levels of lipid peroxide and Iron (p<0.001), whereas, significant decrease in the levels of vitamin-E, Total
Antioxidant Capacity and Total Iron Binding Capacity (p<0.001) was observed. Serum Zinc was significantly increased (p<0.001)
with significant decrease in the levels of copper (p<0.001). Non Significant increase in the activity of Erythrocyte Superoxide
Dismutase (p>0.05) was found in the patients when compared with controls. This suggest that oxidative stress and reduced antioxidant
defense mechanism play an important role in pathogenesis of beta thalassemia major. 相似文献
19.
Neela Patil Vishwas Chavan N. D. Karnik 《Indian journal of clinical biochemistry : IJCB》2007,22(1):45-51
Free radicals play an important role in the pathogenesis of tissue damage in many clinical disorders, including atherosclerosis.
Antioxidants protect the body from damage caused by free radicals. In this study we investigated oxidative stress, antioxidants
and inflammatory molecules in patients with acute myocardial infarction. This study has been carried out on 106 patients with
acute myocardial infarction, (89 men and 17 females). The control group consisted of 50 healthy, age-matched subjects (40
men and 10 females). Levels of Glucose, lipid profile, glutathione reduced, glutathione peroxidase, Superoxide dismutase,
Glycosylated hemoglobin, fibrinogen, vitamin C, vitamin E, malondialdehyde, ceruloplasmin, adenosine deaminase, lysozyme and
sialic acid were measured. Malondialdehyde and ceruloplasmin levels were significantly high and antioxidants such as vitamin
C, vitamin E, glutathione reduced, glutathione peroxidase and superoxide dismutase were significantly decreased in diabetic
and non-diabetic AMI patients as compared with control (p<0.001). Inflammatory markers showed significant rise in diabetic
patients as compared with controls. Our results clearly show increased inflammation and oxidative stress in patients with
acute myocardial infarction. Depression of antioxidant system in these patients confirms this conclusion. 相似文献
20.
Seema Gupta Rajesh Pandey Ranjan Katyal H. K. Aggarwal R. P. Aggarwal S. K. Aggarwal 《Indian journal of clinical biochemistry : IJCB》2005,20(1):67-71
The present study was conducted to evaluate some of the components of antioxidant defense system and oxidative damage in 20
male patients of alcoholic liver disease (ALD). The results were compared with 20 healthy male smokers and 20 healthy male
non-smokers volunteers. Patients were subjected to detailed clinical examination and laboratory investigations. Blood samples
were collected for estimating reduced glutathione (GSH), total thiols (T-SH) malondialdehyde (MDA), transaminases (AST, ALT),
glutathione-S-transferease (GST) and gammaglutamyl transferase (GGT). Serum aspartate amino transferase (AST)/alanine amino
transferase (ALT) ratio was significantly (p<0.01) reduced in ALD patients as compared to the controls. However, the core
of utility of MDA and GST was found to be significantly (p<0.01) increased in ALD patients compared to controls. There was
a significant negative correlation of MDA with both GSH and TSH. Plasma GGT levels were significantly (p<0.01) increased in
alcoholics and the enzyme showed a significant positive correlation with MDA. These results give enough evidence of increased
oxidative stress and compromised antioxidant defense system in patients with ALD. 相似文献