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1.
采用单因子随机分组设计,以真可消化磷(TdP)为指标,配制了5种生长猪试验日粮,并使其总钙与TdP的比例为2∶1.通过对生长性能和血清生化指标等的评定,对TdP需要量进行了初步研究.结果为:(1)得出生长猪平均日增重(ADG)随日粮TdP变化的多元方程;(2)拟合出了料肉比(F/G)随日粮TdP含量变化的多元方程;(3)根据血清无机磷浓度与日粮TdP含量的关系,也拟出了多元方程;(4)血清碱性磷酸酶(ALP)活性随着日粮TdP含量的升高呈下降趋势(P>0·05);(5)血清钙浓度基本不受日粮真可消化磷含量的影响.血清钙浓度随日粮钙含量的变化趋势,与血清无机磷随日粮TdP含量变化的趋势相似,且各组之间差异不显著(P>0·05).由此,本研究推荐生长猪真可消化磷的需要量为0·34%,相应地,总钙的需要量为0·68%.  相似文献   

2.
以"山油4号"油菜幼苗为试材,研究在0%(对照)、4%、8%、12%以及16%PEG-6000(W/V)不同渗透压胁迫下油菜可溶性糖、游离脯氨酸变化量。并研究4%、12%PEG-6000胁迫5d后复水的不同生理反应。通过相关的研究结果看到,PEG浓度的增加和胁迫时间的延长,幼苗中等产生一定的影响,但由于后期重度胁迫下部分苗的枯萎死亡,可溶性糖的含量的变化在第5d后呈下降趋势,但仍高于对照。叶绿素含量4%、8%、12%的处理下,总体呈缓慢上升的趋势,16%PEG胁迫处理呈现先上升后下降的趋势,POD的活性均呈现上升后下降的趋势。胁迫处理后复水,叶片的游离脯氨酸、可溶性糖、不断下降。  相似文献   

3.
管健 《黑龙江科技信息》2014,(4):273-273,112
土地盐碱化是全球性环境问题,在我国也尤为突出。苗木能否在盐碱地上生长,是影响成活的主要原因。本研究通过对经过不同处理的花曲柳各种生理指标的测定,得出不同盐度对生长在同一环境条件下的生理指标的影响,结果表明:盐度增加,叶片水势有明显的降低趋势;苗木随处理盐度的加大,其游离脯氨酸含量逐渐增高,电导率也逐渐升高。  相似文献   

4.
采用水培试验的方法,研究不同浓度铜(0、20、40、60、80和100mg/L)胁迫条件下小叶黄杨的生长和生理特征。结果表明,随铜浓度的增加,小叶黄杨的生物量(鲜重)逐渐下降;蛋白质含量呈下降趋势;叶绿素含量先上升后下降;丙二醛的含量逐渐增加;超氧化物歧化酶和过氧化物酶的活性均呈先增加后下降的趋势。  相似文献   

5.
东北红豆杉幼苗越冬过程中理化特性研究   总被引:1,自引:0,他引:1  
本文研究了4年生东北红豆杉室外初次室外越冬幼苗和自然越冬幼苗在秋冬季节自然降温过程中细胞膜透性、半致死温度、叶绿素含量、可溶性蛋白质含量、可溶性糖、游离脯氨酸含量、SOD和POD的变化情况。揭示了东北红豆杉幼苗适应低温条件的生理生化特性,得出了初次室外越冬幼苗的理化指标显示其对低温的耐受力远低于多次自然越冬的幼苗。  相似文献   

6.
以金荞麦叶和覆盆子叶为植物原料,经酵母菌发酵制备农用酵素营养液,研究其发酵过程中主要营养组分的动态变化规律。结果表明,随着发酵时间的延长,金荞麦叶和覆盆子叶农用酵素营养液各营养组分的变化趋势类似。游离氨基酸含量呈先上升后下降趋势;总氮含量呈不规则变化;总酚、总酸和总磷含量总体呈上升趋势;总糖含量呈下降趋势;钾离子含量呈先升高后下降再上升变化。但两者养分含量除钾离子外差异较为明显。金荞麦叶农用酵素营养液的总氮、游离氨基酸以及总酸含量高于覆盆子叶农用酵素营养液;而覆盆子叶农用酵素营养液的总糖、总磷和总酚含量较高。  相似文献   

7.
本文采取实验研究的方式,将亚洲百合作为研究对象,对其不同发育期生理变化进行研究分析,主要研究干物率、可溶性糖、蛋白质含量、淀粉等物质的变化情况。在研究中,主要围绕鳞茎和茎叶开展,并对两者之间含量变化进行对比分析。经过一系列的实验研究,发现百合花的茎叶存在干物率上升、淀粉含量下降、糖分变化小、蛋白质开花期含量最大的特点,鳞茎则具有干物率上升、淀粉上升、糖分下降、蛋白质下降的现象。在营养物质增加的同时,PPO、POD都会上升,并且随着生长发育其活性不断下降。  相似文献   

8.
在西藏裸大麦拔节期,干旱胁迫导致叶片叶绿素和可溶性蛋白质含量下降,细胞膜透性增加,不同叶位比较,以下部叶片受害最大,经分析表明,干旱胁迫下叶片中O^-2和H2O2逐渐累积,膜脂过氧化加剧,导致叶片细胞的氧化伤害。  相似文献   

9.
目的:大黄久煎其主要成分含量的变化。方法:以大黄中蒽醌和总鞣质两个主要成分为分析对象,采用紫外分光光度(UV)法分别测定大黄在不同煎煮时间中蒽醌类成分和总鞣质的含量变化情况。结果:总鞣质成分含量在规定煎煮时间内一直上升,结合蒽醌含量先上升后稍微下降,游离蒽醌一直上升,总蒽醌含量保持基本不变。结论:结合蒽醌在煎煮过程中转化成游离蒽醌,且久煎大黄泻下力减弱的主要是由于收涩类成分总鞣质的大量游离造成的。  相似文献   

10.
李小安 《青海科技》2011,18(2):40-42
在低温胁迫(-4℃)及不同时间长度(24h、48h、72h)下,以扁蓿豆种子(青藏扁蓿豆、内蒙古直立型扁蓿豆、青海匍匐型扁蓿豆、盐池扁蓿豆和吉林扁蓿豆)5个样品为材料,研究了扁蓿豆种子内游离脯氨酸和可溶性糖含量的变化规律。结果表明:在低温胁迫下,5个品种扁蓿豆种子内游离脯氨酸和可溶性糖的绝对含量都是呈现出上升的趋势,从而证明扁蓿豆种子是通过提高游离脯氨酸和可溶性糖的绝对含量来适应低温逆境、减轻低温伤害的,也进一步证明游离脯氨酸和可溶性糖可作为植物抗寒性的一项生理生化指标。  相似文献   

11.
采用PEG-6000模拟干旱胁迫研究表明,干旱胁迫后不同水、陆稻品种茎叶所受生长抑制强于根系所受抑制,但是旱稻品种巴西陆稻对干旱逆境具有较强的生长适应能力,与常规稻品种相比,旱稻品种巴西陆稻所受的生长抑制相对较小。干旱胁迫条件下,不同水陆稻品种叶片中可溶性糖(SS)含量、游离氨基酸总量(TFA)、游离脯氨酸(Pro)含量、POD活性、CAT活性均呈一致的上升趋势,且旱稻品种巴西陆稻具有较强的有机渗透调节能力和酶促抗氧化能力,干旱胁迫后其叶片中各相应指标的变化幅度均显著高于常规稻品种。  相似文献   

12.
The root of Glycyrrhiza glabra is a traditional medicine used mainly for the treatment of peptic ulcer, hepatitis C, pulmonary and skin diseases, although clinical and experimental studies suggest that it has several other useful pharmacological properties such as antiinflammatory, antiviral, antimicrobial, antioxidative, anticancer activities, immunomodulatory, hepatoprotective and cardioprotective effects. Glycyrrhizinic acid, a major component of licorice, has antiulcer effect by raising the local concentration of prostaglandins that promote mucous secretion and cell proliferation in the stomach. Glycyrrhizin shows hepatoprotective effect by preventing changes in cell membrane permeability, inhibiting phospholipase A2 (PLA2) and increasing survival rate of hepatocytes. Glabridin has effect in melanogenesis and inflammation by inhibiting the tyrosinase activity of melanocytes. α-glycyhrritinic acid exhibits anti-inflammatory activity by inhibiting glucocorticoid metabolism. In present study ethanolic (95%) extract of root of Glycyrrhiza glabra and its fractions were investigated for its antidyslipidaemic activity on HFD induced dyslipidaemic hamsters. Ethanolic extract and its ethyl acetate soluble, water soluble and hexane soluble fractions decreased serum level of total cholesterol by 25.9, 38.0, 39.0 and 26.3%, respectively. On the other hand ethanolic extract, ethyl acetate soluble, water soluble and hexane soluble fraction increased the serum HDL-cholesterol level by 14.8, 34.3, 27.3 and 17.2%, respectively. Ethanolic extract, ethyl acetate fraction, aqueous fraction and hexane fraction decreased triglyceride level by 31.3, 37.2, 41.2 and 28.9%, respectively. The reduction in LDL-cholesterol level by ethanolic extract, ethyl acetate soluble fraction and water soluble fraction were 43.9, 31.0, 33.4 and 24.6%, respectively.  相似文献   

13.
BackgroundIn biodegradation processes free enzymes often undergo deactivation. Thus, it is very important to obtain highly stable enzymes by different methods. Immobilization allows for successful stabilization of many multimeric enzymes by increasing the rigidity of the enzyme structure. This study aimed to evaluate some environmental factors that affect catechol 1,2-dioxygenase from Stenotrophomonas maltophilia KB2 immobilized in alginate hydrogel. The goal of the present work was to improve the functional stability of the enzyme by increasing its structural rigidity.ResultsImmobilization yield and expressed activity were 100% and 56%, respectively. Under the same storage conditions, the activity of the immobilized enzyme was still observed on the 28th d of incubation at 4°C, whereas the free enzyme lost its activity after 14 d. The immobilized enzyme required approximately 10°C lower temperature for its optimal activity than the free enzyme. Immobilization shifted the optimal pH from 8 for the soluble enzyme to 7 for the immobilized enzyme. The immobilized catechol 1,2-dioxygenase showed activity against 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, and 3,5-dichlorocatechol. The immobilization of the enzyme promoted its stabilization against any distorting agents: aliphatic alcohols, phenols, and chelators.ConclusionsThe entrapment of the catechol 1,2-dioxygenase from S. maltophilia KB2 has been shown to be an effective method for improving the functional properties of the enzyme. Increased resistance to inactivation by higher substrate concentration and other factors affecting enzyme activity as well as broadened substrate specificity compared to the soluble enzyme, makes the immobilized catechol 1,2-dioxygenase suitable for the bioremediation and detoxification of xenobiotic-contaminated environments.  相似文献   

14.
BackgroundInferior Tieguanyin oolong tea leaves were treated with tannase. The content and bioactivity of catechins in extracts from the treated tea leaves were investigated to assess the improvement in the quality of inferior Tieguanyin oolong tea.ResultsAnalysis showed that after treatment, the esterified catechin content decreased by 23.5%, whereas non-galloylated catechin and gallic acid contents increased by 15.3% and 182%, respectively. The extracts from tannase-treated tea leaves showed reduced ability to bind to BSA and decreased tea cream levels. The extracts also exhibited increased antioxidant ability to scavenge OH and DPPH radicals, increased ferric reducing power, and decreased inhibitory effects on pancreatic α-amylase and lipase activities.ConclusionsThese results suggested that tannase treatment could improve the quality of inferior Tieguanyin oolong tea leaves.  相似文献   

15.
16.
不同药剂防治水稻二化螟的田间试验   总被引:1,自引:0,他引:1  
筛选出高效低毒的药剂替代高毒农药防治水稻二化螟田间试验,结果表明:5%锐劲特悬浮剂40ml/667m2、98%巴丹可溶性粉剂50g/667m2、48%毒死蜱乳油100ml/667m2叶面喷雾对水稻二化螟具有较好的防治作用,且总体防治效果优于其他药剂处理。其防治效果分别达到100%、96.0%和91.3%,增产率分别为10.5%、15.9%和9.5%,且对水稻安全。  相似文献   

17.
作者曾经证明香榧叶中具有特征成分榧黄素(kayaflavone)。最近,又从香榧叶中分得一 个新的二萜特征成分香榧酯(torreyagrandate),为了获得更多的特征成分,我们自榧树叶中共鉴  定出26个精油成分。从组成比看,其特点是苧烯占优势(44.24%),其次为 α-蒎烯(20.75%),再次为δ-3-蒈烯(4%)。叶中所含的榧烯醇(torreyol)为特征成分。  相似文献   

18.
Ocimum sanctum leaves have been traditionally used in treatment of diabetes mellitus. Dietary supplementation of fresh tulsi leaves in a dose of 2 gm/kg BW for 30 days led to significant lowering of blood glucose levels in test group. Intake ofOcimum sanctum also led to significant increase in levels of superoxide dismutase, reduced glutathione and total thiols, but marked reduction in peroxiodised lipid levels as compared to untreated control group. The leaves were found to possess both superoxide and hydroxyl free radical scavenging action. The present observations establish the efficacy ofOcimum sanctum leaves in lowering blood glucose levels and antioxidant property appears to be predominantly responsible for hypoglycemic effect.  相似文献   

19.
丹贝(Tempe)发酵过程中的成分变化及其功能性   总被引:1,自引:0,他引:1  
丹贝(Tempe)是一种传统的印度尼西亚发酵豆制品,比未发酵豆制品具有更多的生物活性成分.这主要是由于丹贝在发酵过程中发生了一系列的生物化学变化,主要体现在游离氨基酸、游离脂肪酸、B族维生素及生育酚、可溶性矿物质含量的增加,活性异黄酮苷元、SOD的产生,抗营养因子和胀气因子的有效去除.文章对发酵过程中的成分变化进行了具体综述,并对Tempe的功能性及其产品开发进行了探讨.  相似文献   

20.
分光光度法测定蒙古栎叶中多酚的含量   总被引:2,自引:0,他引:2  
以没食子酸为标准品,使用福林酚试剂(FC),通过分光光度法,对蒙古栎树叶中植物多酚含量进行了测定.首先从方法的稳定性、线性关系、精确性和准确性角度,对FC法进行了调整,确定了以没食子酸为标准的最佳反应条件.结果表明,最佳反应条件为0.15mL FC试剂(1mol/L),0.15mL 10%体积分数的Na2CO3,25℃反应80min后于760nm处测吸光值.使用调整后的方法测得结果为:蒙古栎叶中总酚含量为6.39%,RSD为1.90%.方法操作简单,结果重现性好, 可用来测定蒙古栎叶中总酚的含量.  相似文献   

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