共查询到19条相似文献，搜索用时 217 毫秒
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《昆明师范高等专科学校学报》2014,(6)
为探明弥勒市8个主栽葡萄品种的亲缘关系,研究以这8个葡萄品种为材料,利用ISSR分子标记技术进行基因组多态性检测,从40条ISSR随机引物中筛选出10条用于ISSR扩增.结果显示,筛选出的这10条引物共扩增出81条谱带,其中66条为多态性谱带,多态性百分率较高,为79.9%;根据ISSR的扩增结果进行相似性系数分析,8个葡萄品种的遗传相似性系数在0.50~0.78之间,平均为0.647;通过UPGMA进行聚类分析,在遗传相似性系数为0.58处,8份试材分为2大类群,其中第1大类群包含3份欧美杂种品种和1份未知来源品种,第2大类群包含4份欧亚种品种.由此推测未知来源的这一品种属于欧美杂交种品种. 相似文献
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《南阳师范学院学报》2018,(3):21-25
利用ISSR标记对14个月季品种进行遗传多样性分析.正交设计确定20μL最佳ISSR-PCR反应体系,筛选7条引物对14个月季品种的基因组DNA进行ISSR-PCR扩增.7条引物共扩增出60条DNA条带,多态性位点52个,多态性位点百分率为86.0%,品种间遗传一致度为0.500.85.UPGMA聚类显示,藤本月季与大花月季和微型月季存在不同程度的亲缘关系.月季品种间的遗传多样性较高,大花月季、藤本月季和微型月季的品种类群间可能发生过杂交和基因交换. 相似文献
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毛加宁 《乐山师范学院学报》2008,23(5):32-34
利用RAPD技术,从248个随机寡核苷酸（10bp）中筛选出18个引物能在供试的Ⅱ优系杂交水稻及部分亲本间扩增出51条稳定性较好的多态性片段。其中有6个引物能在供试材料间稳定地扩增出19个强的多态性标记。利用这些标记能有效地区分各组合中不育系、保持系、恢复系和F1,并能看出各组合中不育系与保持系、不育系与恢复系、F1与亲本间的遗传关系。 相似文献
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《南阳师范学院学报》2019,(6):32-36
采用目标起始密码子多态性(SCoT)技术来分析16个月季品种的遗传多样性.通过对SCoT-PCR反应体系优化和PCR扩增程序探索,从55条SCoT分子标记引物中筛选出9条引物.品种间成对遗传相似度变幅介于0.400 0～0.775 0,9条SCoT标记引物的扩增多态性位点百分率为93.5%,显示月季品种间具有较丰富的遗传多样性.分析UPGMA聚类结果可知:16个月季品种在遗传相似性系数为0.62处被划分成2个聚类分支,第一分支又分为三个亚分支,显示不同月季品种间具有明显的遗传分歧,遗传关系复杂.大花月季、丰花月季与微型月季的亲缘关系相对较近,推测月季品种在驯化进程中存在基因重组和基因渐透现象;藤本月季进化独立,与其他园艺类群月季存在较明显的遗传分歧. 相似文献
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王子迎 《安徽教育学院学报》2005,23(6):94-96,105
采用随机扩增多态性DNA(RAPD)和简单序列重复区间扩增多态性(Inter-simple sequence repeat,ISSR)技术,利用12条RAPD引物和10条ISSR引物对10个安徽香菇菌株进行了DNA标记比较分析.结果表明,ISSR-PCR较RAPD-PCR稳定,更具可操作性;供试菌株存在比较显著的遗传变异,具有较丰富的遗传多样性. 相似文献
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在220个随机引物中,筛选出6个具有较高多态性检测能力的引物．用这6个随机引物对30份杂交水稻亲本材料进行了RAPD分析,共检测到56条多态性带．聚类分析表明,不同亲本材料可以被较为明确地区分,显示了应用RAPD技术鉴定稻种具有简便、灵敏、准确的特点及良好的应用前景．本研究对影响RAPD技术稳定性的因素进行探讨,并提出了克服这些不利因素的技术措施． 相似文献
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使用合丰25等来自13个育种单位的13份大豆(Glycine max)品种对大豆20个连锁群上的100对SSR标记进行筛选,最终保留了扩增稳定且多态性较高的43对SSR标记,分析了黑龙江省83个主栽大豆品种的遗传多样性.结果表明,在所有供试材料中共鉴定出等位变异157个,每个位点2-7个,平均为3.65个.品种间遗传相似系数为0.216-0.937,平均为0.638 4,表明黑龙江省大豆品种的遗传相似性较大.故拓宽黑龙江省大豆品种的遗传基础具有重要意义. 相似文献
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胡桃楸ISSR-PCR反应体系的建立及优化 总被引：3，自引：0，他引：3
在研究胡桃楸遗传多样性的过程中,为了获得清晰、重复性好的ISSR扩增结果,采用改良的CTAB法提取胡桃楸基因组DNA,利用正交设计与单因子试验相结合的方法对影响胡桃楸ISSR-PCR反应体系的5个主要因素(Taq酶、Mg~(2+)、dNTP、引物、模板DNA)在4个水平上进行优化设计。通过综合比较分析,筛选出各反应因素的最佳水平,建立胡桃楸ISSR-PCR最佳反应体系:20μL的反应体系中,Taq酶1.0 U,Mg~(2+)2.0 mmol/L,dNTP 0.2 mmol/L,模板DNA 50～250 ng,引物0.4μmol/L。在此基础上筛选出13条多态性好、扩增稳定的ISSR引物,并确立了最佳退火温度和循环次数。这一优化系统的建立为今后利用ISSR技术进行胡桃楸遗传多样性、亲缘关系以及物种保护等的研究奠定了基础。 相似文献
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Manuel TORAL IBAEZ Margarita CARU Miguel A.HERRERA Luis GONZALEZ Luis M.MARTIN Jorge MIRANDA Rafael M.NAVARRO-CERRILLO 《Journal of Zhejiang University. Science. B》2009,10(2)
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin. 相似文献
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Manuel Toral Ibañez Margarita Caru Miguel A. Herrera Luis Gonzalez Luis M. Martin Jorge Miranda Rafael M. Navarro-Cerrillo 《Journal of Zhejiang University. Science. B》2009,10(2):112-119
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene
diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied.
The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.
The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis
in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and
were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index
and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).
Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of
49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic
variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a
geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although
they are derived from the same geographic origin.
Project (No. CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile 相似文献
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Umezuruike Linus OPARA Dan JACOBSON Nadiya Abubakar AL-SAADY 《Journal of Zhejiang University. Science. B》2010,11(5):332-341
Banana is an important crop grown in Oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs.This study employed amplified fragment length polymorphism(AFLP) to investigate the genetic variation in local banana cultivars from the southern region of Oman.Using 12 primer combinations,a total of 1094 bands were scored,of which 1012 were polymorphic.Eighty-two unique markers were identified,which revealed the distinct separation of the seven cultiva... 相似文献
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Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding 总被引：1，自引：0，他引：1
Jun-mei Wang Jian-ming Yang Jing-huan Zhu Qiao-jun Jia Yue-zhi Tao 《Journal of Zhejiang University. Science. B》2010,11(10):792-800
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR)
genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A
total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus
ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based
on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley
varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley
varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing
elite genotypes from different origins for malting barley breeding in China. 相似文献
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基于杨梅基因组鸟枪法测序开发的107个SSR标记及其在品系鉴定中应用 总被引：2，自引：0，他引：2
Hui-min JIA ;Yu-tong SHEN ;Yun JIAO ;Guo-yun WANG ;Xiao DONG ;Hui-juanJIA ;Fang DU ;Sen—miaoLIANG ;Chao—chao ZHOU ;Wei—huaMAO ;Zhong-shan GAO 《Journal of Zhejiang University. Science. B》2014,15(11):997-1005
研究目的：开发多态性高的基因组简单重复序列（SSR）标记,为杨梅遗传多样性、遗传图谱构建及品种鉴定相关研究奠定基础。
创新要点：从杨梅全基因组鸟枪法测序数据中新开发了107个SSR标记,并应用于品种间遗传多样性分析及品系鉴定。
研究方法：应用生物信息学软件从基因组测序数据中搜索SSR位点并设计相应引物,添加通用的M13尾巴和荧光标识筛选多态性高的SSR标记。
重要结论：开发的107个基因组SSR标记可以广泛应用于杨梅种质资源和遗传育种研究,鉴定出三个杨梅新品系。 相似文献
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线粒体DNA具有突变速度快、重组率低、严格母系遗传、拷贝数多等特点,近年来成为分子考古研究中的一个热点。为了研究古墓群中的血亲关系,可以从古墓葬遗留骨骸、牙齿中提取和扩增mtDNA片断进行分析。通过设计2对部分重叠的引物,扩增线粒体16055-16218区域目标片段,进行反相高效液相色谱分析,确认四个样品对应片断在反相柱上的保留时间上存在差异,以此推断扩增片段在序列上可能存在差异。为利用mtDNA分析人类母系血缘关系奠定了基础。 相似文献
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