幼师生心理危机预警模型研究     

林琳 《科教文汇》2011,(35):197-197,208

幼师生心理危机是影响学校稳定和发展的重要因素。针对预警在幼师生心理危机管理中的重要性,结合心理危机产生的原因,提出了幼师生心理危机预警指标体系。考虑到指标信息的模糊性,引入多层次模糊综合评判方法,建立了幼师生心理危机预警模型,以期在实践中得到应用。  相似文献   

Fabrication of a gel particle array in a microfluidic device for bioassays of protein and glucose in human urine samples     

Lin L  Gao Z  Wei H  Li H  Wang F  Lin JM 《Biomicrofluidics》2011,5(3):34112-3411210

This paper describes a simple method for fabricating a series of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures inside microfluidic channels as probe for proteins and glucose. In order to demonstrate the feasibility of this newly developed system, bovine serum albumin (BSA) was chosen as a model protein. PEG microcolumns were used for the parallel detection of multiple components. Using tetrabromophenol blue (TBPB) and the horseradish peroxidase/glucose oxidase reaction system, bovine serum albumin (BSA) and glucose in human urine were detected by color changes. The color changes for BSA within a concentration range of 1-150 μM, and glucose within a range of 50 mM-2 M could be directly distinguished by eyes or precisely identified by optical microscope. To show the practicability of the gel particle array, protein and glucose concentrations of real human urine samples were determined, resulting in a good correlation with hospital analysis. Notably, only a 5 μL sample was needed for a parallel measurement of both analytes. Conveniently, no special readout equipment or power source was required during the diagnosis process, which is promising for an application in rapid point-of-care diagnosis.  相似文献   

Label-free microfluidic characterization of temperature-dependent biomolecular interactions     

Nguyen T  Pei R  Landry DW  Stojanovic MN  Lin Q 《Biomicrofluidics》2011,5(3):34118-341187

We present a microfluidic approach to characterizing temperature-dependent biomolecular interactions. Solvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Unbound AVP were collected and analyzed with matrix-assisted laser desorption∕ionization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36–45 °C) or dissociation (25–33 °C and 50–65 °C) occurs. Additionally, temperature-dependent binding isotherms were constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasopressin-spiegelmer interaction.  相似文献   

Stem cells in microfluidics     

Wu HW  Lin CC  Lee GB 《Biomicrofluidics》2011,5(1):13401

Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.  相似文献   

奥林匹克主义的历史哲学审视——兼评迪卡娅·凯泽斯塔提乌的“当代奥林匹克主义意识形态的本质变迁”     

王润斌  杨麟  熊晓正 《体育科学》2011,31(8):3-19

迪卡娅·凯泽斯塔提乌把奥林匹克主义看作一种被顾拜旦、卡尔.蒂姆、奥林匹克学院等"代理人"不断阐释与建构的元叙事哲学。在评述其博士论文并对奥林匹克主义进行历史哲学审视的基础上认为,立足批判现实主义的立场,以现代性、文化帝国主义、全球化理论为视角,运用质性研究范式,方可深刻阐释倡导普世伦理的奥林匹克主义与地缘政治、社会文化、经济博弈中的哲学议题。并指出多元文化主义是奥林匹克主义未来走向的必由之路。  相似文献   

TLR4信号通路参与有氧运动对ApoE基因敲除小鼠血管炎性损伤的修复     

陈嘉勤  柳维林  张川湘  王茂材  李林 《体育科学》2011,31(8)

目的:通过中小强度有氧运动和辛伐他汀药物干预,探讨AS血管炎性损伤的修复及机制。方法:应用ApoE基因敲除和高脂高胆固醇饲料诱发小鼠AS,分为动脉硬化组、运动组、用药组;ELISA测定血清炎性因子及激素水平;免疫组化法阳性标记TLR4、NF-κb、EGF、TGF-β;RT-PCR测定CRP、MCP-1 mRNA表达。结果:12周动脉硬化组ApoE基因敲除小鼠血管可见明显斑块组织;用药组内皮表层细胞肿胀呈泡沫样,运动组则未见以上组织改变;运动和用药组小鼠血清IL-6、TNF-a含量和血管组织TLR4、NF-κb、CRP、MCP-1表达均降低(P<0.05),而血清IL-10、ET1、AngⅡ和内皮细胞EGF、TGF-βmRNA表达均升高(P<0.05)。结论:有氧运动有效防治AS小鼠血管炎性损伤,其可能机制是介导了Toll样受体通路并恢复血管激素分泌与促进内皮细胞再生。  相似文献   

科研合作对高校学科发展的影响分析——基于计算机学科国际一流高校的实证     

张发亮  林约佩  薛雨纤 《情报探索》2021,(1):33-40

[目的/意义]科研合作与交流是现代学科发展的重要特征,分析其对高校学科发展的影响,有利于指导高校选择和改进合作策略,推动我国一流学科建设。[方法/过程]利用科学计量、社会网络分析等方法,基于学科论文数据,从作者、机构和国家合作程度、合作机构等级和合作网络等层面分析和测度高校学科的科研合作特征。而后建立多元线形回归模型分析各特征对高校学科的后期知识生产力、影响力和质量的实质影响,并以计算机学科10所国内外一流高校的数据进行实证。[结果/结论]不同科研合作特征对高校学科的发展具有不同的影响。合作网络中的地位和国际合作程度对学科知识生产力的促进作用最为明显;而机构和国际合作程度对学科知识质量和整体影响力的提升更为关键。我国高校应重点加强外部机构合作、特别是高等级机构合作和国际合作,以提升学科知识质量和整体水平。  相似文献   

自有知识、知识溢出与区域创新产出     

苏屹  林周周 《科研管理》2021,42(1):168-176

立足于区域边界的内外部视角,将创新活动的知识资源划分为自有知识和知识溢出两部分,利用2009—2016年中国30个省（市、自治区）的面板数据,运用OLS和分位数回归方法,实证分析了自有知识、知识溢出对区域创新产出的影响。OLS回归结果表明：自有知识对区域创新产出具有显著正向影响,而知识溢出的影响显著为负;自有知识的主效应大于知识溢出,且二者在影响区域创新过程中存在显著的协同效应。分位数回归结果表明：随着分位点的提高,自有知识对区域创新产出的促进效应先升后降;知识溢出对区域创新产出除在0.25分位点没有显著影响外,在其他分位点均存在显著负向影响;自有知识与知识溢出的交互效应在区域创新产出不同分位点的影响不一致。  相似文献   

用创新生态理论和“四链”融合研究建设粤港澳大湾区国际科技创新中心     

杨明  林正静 《科技管理研究》2021,41(13):87-93

以创新生态系统理论为基础,构建一 个既能反映国际科技创新中心动力来源、又能协调创新要素与创新环境之间相互关系的"四链"融合发展的理论框架,这是一种跨组织共生式协同创新发展新范式.其核心是由科技导向型产业链、科技创新链、科技资金链、科技服务链构成的"四链",通过相互融合、相互作用,优化创新生态系统,提高区域创新效率,协同建设高水平的国际科技创新中心.基于这个理论框架,在对现实基础和制约因素进行客观研判的基础上,提出建设粤港澳大湾区国际科技创新中心的对策建议.  相似文献   

基于结石体微观特征的全风化花岗岩防渗加固机理研究     

陈裕  郑洪  曹函  林飞  王旭斌 《科技通报》2021,37(3):22-29

工程上常用注浆的方法对软弱基岩进行加固,为了探究郴州水库全风化花岗岩注浆防渗加固的可行性以及其加固机理,本文采用X-RD衍射仪、SEM扫描电镜和贝世德全自动比表面及孔径分析仪研究了全风化花岗岩的结石体微观结构特征.结果表明:注浆后岩体的矿物成分发生显著变化;岩体微观结构由粒状架空结构提升至为缝合线式、镶嵌式结构;岩体微孔的形态和数量变化趋势直观的反映了风化地层内部结构的变化及防渗加固效果.该研究结果可为全风化岩体的注浆效果评价和注浆工艺的提升提供理论指导.  相似文献