Ebselen对大强度耐力训练大鼠肝脏细胞凋亡及自由基代谢的影响   总被引：3，自引：1，他引：2  

熊正英  刘军 《体育科学》2008,28(11)

目的:探讨ebselen对运动训练大鼠肝细胞凋亡表达、自由基代谢等指标的影响,为其在运动医学领域的应用提供实验依据。方法:30只SD雄性健康大鼠,随机分为3组:安静对照组(A)、运动对照组(B)和运动加药组(C),每组10只。将ebselen溶于5%的DMSO生理盐水溶液中,用量为50mg/kg/d,灌胃给运动加药组,其他两组灌服给同等量的DMSO生理盐水作为对照。建立为期8周的大强度耐力训练模型。常规方法检测大鼠肝脏MDA、TAOC、SOD、GSH-Px等指标,免疫组化法检测凋亡蛋白Bcl-2、Bax的表达;TUNEL检测肝细胞凋亡指数;透射电子显微镜观察肝细胞超微结构的改变。实验数据由SPSS11.0统计软件进行处理。结果:与安静组相比,运动组大鼠肝脏内MDA含量显著升高,抗氧化酶SOD、GSH-Px均下降,T-AOC下降,细胞凋亡指数AI显著增高,Bax/Bcl-2比值下降,肝细胞超微结构损伤明显;加入ebselen进行干预后,大鼠抗氧化酶SOD、GSH-Px均有所升高,MDA含量显著下降,总抗氧化能力显著升高,细胞凋亡指数AI下降,Bax/Bcl-2比值上升,肝细胞超微结构损伤被有效抑制。结论:Ebselen对运动训练导致的肝细胞损伤有明显的保护作用,机制可能与ebselen可以抑制运动诱导的肝细胞凋亡、提高大鼠肝脏抗氧化酶表达等有关。  相似文献   

耐力运动对非酒精性脂肪肝小鼠肝脏NO 水平、NOS 活性及表达的影响   总被引：1，自引：0，他引：1  

马国栋  刘艳环 《天津体育学院学报》2008,23(6):516-518

目的:研究耐力运动对非酒精性脂肪肝小鼠肝脏NO水平、NOS活性及表达的影响。研究方法:雄性8周龄ApoE基因敲除小鼠24只,西方膳食14周后,随机分为2组,非酒精性脂肪肝模型组(H,n=12,14周),跑台运动组(E,n=12,14 12周)。跑台运动12周,坡度为0°,跑速13m/min,60min/time,每周5次。在造模与训练结束后,测定肝脏总NOS、cNOS、iNOS酶活性、NO水平及eNOS和iNOSmRNA的表达,光镜观察脂肪肝病变程度。结果:E组肝脏总NOS、iNOS酶活性和NO水平显著高于H组,cNOS未见显著性改变;E组eNOS和iNOSmRNA表达显著高于H组;E组肝脏实质细胞脂肪滴明显减少。结论:耐力训练在显著降低非酒精性脂肪肝肝脏脂肪滴的同时,伴随着肝脏NO含量、总NOS活性和iNOS活性的显著增加以及eNOS和iNOSmRNA的表达上调,提示运动导致NO、NOS活性和表达的升高在非酒精性脂肪肝发病过程中可能具有重要的生理意义。  相似文献   

长跑运动员疲劳特点及体能恢复     

王文胜 《军事体育进修学院学报》2007,26(2):121-123

提高长跑运动员的运动成绩，提高运动员训练质量，把握训练规律，增强训练科学性和规律性，就长跑运动员训练而出现的疲劳特点及相对疲劳的体能恢复进行探讨。  相似文献   

新疆少数民族体育女教师职业倦怠的调查研究     

刘卫东  徐万彬 《吉林体育学院学报》2007,23(3):29-30

体育教师是职业倦怠感的高发人群,采用问卷调查法研究新疆少数民族体育女教师的职业倦怠现状和影响因素.结果表明:少数民族体育青年女教师比中老年教师的职业倦怠状况严重,心理落差及个人心理承受能力是造成职业倦怠的主要因素,并随着学校层次的提升职业倦怠有下降趋势.  相似文献   

耐力性运动防治血脂异常的机制   总被引：1，自引：0，他引：1  

张培珍  田野 《西安体育学院学报》2007,24(2):66-69

血脂异常是冠心病的首要危险因素,因此,如何通过安全有效的方式防治血脂异常,进而防治冠心病一直是学术界研究的热点。运动疗法作为防治血脂异常的安全有效的手段,正日益受到国内外专家学者的关注。  相似文献   

有氧耐力运动对老年人免疫功能的影响     

杨瑾  熊正英 《山东体育学院学报》2004,20(1):47-49

老年人随着衰老免疫功能下降，体内T淋巴细胞、B淋巴细胞、免疫球蛋白、自然杀伤细胞等均发生变化，适量的有氧耐力运动可以改善老年人机体免疫功能。  相似文献   

上海女足运动员专项速度耐力测试方法的研究          下载免费PDF全文

王晨  林志桦  孙琦敏 《体育科研》2008,29(4):72-73，66

通过实验室、场地速度耐力相关指标的测试和相关因素的分析，检验场地测试速度耐力方法的可靠性，为上海女运动员的体能测试组中速度耐力测试提供科学、有效的方法。结果显示：30m疲劳测试方法可以作为上海女足运动员的专项速度耐力测试方法，平均速度这一指标可以有效地反映上海女足运动员的专项速度耐力，该方法简单可靠，可操作性强，可以为上海女足运动员的专项速度耐力训练效果的评定提供科学的量化标准。  相似文献   

高原耐力训练中抗高原反应能力关系研究     

姚明义 《科技通报》2015,(2):22-24

目的:研究通过高原耐力训练,生成体内血胰岛素,并建立起与抗高原反应能力关系模型,评估人体在高原等恶劣环境下训练的生理机能。方法:研究对象为本校篮球队在青藏高原驻训的23名篮球队员,采用SPSS 13.0统计软件,测得运动员体内产生的血胰岛素聚合物Pluronic-b-PCL(Entry 0)和Pluronic-b-P(CL-co-BCL)的显著差异性,从而分析与抗高原反应能力的关系。引入有助于提高抗高原反应的半乳糖分子,通过DLS测试粒子表面的zeta电位值,分析受测运动员的调节纤维激酶值。结果:在阳离子型嵌段共聚物P(PEGMEMA16-co-PEGMA7)-b-PDMAEMA53中N/P比分别为0.3,0.6,0.8,1.5,2.5,3.0,8.0,受测运动员的生成的P(PEGMEMA-co-PEGMA)-b-PDMAEMA为2.155 g,0.1 mmol,PMDETA(104μL,0.5 mmol),Cu Cl(0.0258 g,0.25 mmol),在运动后24 h,血胰岛素与抗高原反应能力相关系数有所增大,运动后48 h,血胰岛素生成与抗高原反应能力相关系数有所较小,同时BP与调节纤维激酶相关系数增大,抗高原反应能力显著提高(P0.05)。结论:通过高原耐力训练,逐步增加红细胞和血红蛋白的生成,促进血胰岛素生成,改善血液输送氧的能力,使新进入高原的人发挥他正常的生理效能,指导高原训练和高原反应病症的治疗和预防。  相似文献   

Pacing profiles and pack running at the IAAF World Half Marathon Championships     

Brian Hanley 《Journal of sports sciences》2015,33(11):1189-1195

The aim of this study was to describe the pacing profiles and packing behaviour of athletes competing in the IAAF World Half Marathon Championships. Finishing and split times were collated for 491 men and 347 women across six championships. The mean speeds for each intermediate 5 km and end 1.1 km segments were calculated, and athletes grouped according to finishing time. The best men and women largely maintained their split speeds between 5 km and 15 km, whereas slower athletes had decreased speeds from 5 km onwards. Athletes were also classified by the type of packing behaviour in which they engaged. Those who ran in packs throughout the race had smaller decreases in pace than those who did not, or who managed to do so only to 5 km. While some athletes’ reduced speeds from 15 to 20 km might have been caused by fatigue, it was also possibly a tactic to aid a fast finish that was particularly beneficial to medallists. Those athletes who ran with the same competitors throughout sped up most during the finish. Athletes are advised to identify rivals likely to have similar abilities and ambitions and run with them as part of their pre-race strategy.  相似文献   

The Role of Endurance Contests in the Construction of Authority and Social Order in Rural China: Cases in the Qing Dynasty and the Republic of China     

Guohua Zheng 《国际体育史杂志》2015,32(8):1057-1070

By investigating four cases from the Qing Dynasty (1622–1911) and from the Republic of China (1911–1949), it was found that inter-village disputes normally arose because of uneven distribution of public resources in China's rural society. To settle the disputes, villagers tended to apply civil mediation, normally conducting endurance contests, rather than seek governmental arbitration. The authority and social order was thereby based on endurance contests. Conducting such contests was certainly advantageous to larger villages in their quest for more public resources. However, it simultaneously restricted their privatization of public resources, as the smaller villagers were able to equally raise their voices to resist them and to obtain their own public resources by proposing and participating in endurance contests. Although originally it was an unfair way of settling the disputes over uneven distribution of public resources in rural China, it gave hope to the disadvantaged to protect their own rights and interests at a time when governmental arbitration could not be relied upon.  相似文献