蟋蟀基因组DNA提取方法     

卢祥云  王立新  王芝云  刘素青 《常熟理工学院学报》2002,16(2):44-46

对直翅目蟋蟀总科部分种类酒精浸泡标本的基因组DNA进行了提取，样品经检测，谱带基本呈线形，无拖尾；OD(260／280nm)值86．11％在1．6—1．8之间。  相似文献   

鸡场禽流感的诊断     

程喜荣 《黄冈职业技术学院学报》2002,4(4):59-63

本实验对某鸡场鸡群中出现的一种以呼吸道症状和产蛋下降为主要特征的疫情进行了临床症状和流行病学调查,对病死鸡进行了解剖检查并从病鸡病料中分离到一种病毒,通过血凝(HA)和血凝抑制(HI)试验、病毒中和试验(VNT)和琼脂免疫扩散试验(AGP)等方法对该病毒进行了签字。结果表明该次疫情为A型流感病毒感染。  相似文献   

基于网形的配电网故障判断、隔离与恢复     

齐宝平  李友海 《安康学院学报》2005,17(3):59-63

介绍了基于网形的故障区域判断方法,并论述了配电开闭所和馈线协调配合的故障判断和隔离,给出故障后健全区域优化恢复的方案.  相似文献   

棒纹牛蛭体内抗凝血蛋白的提取     

仝向荣王德斌  马绍宾 《昆明师范高等专科学校学报》2004,26(4):70-72

通过离子交换、凝胶过滤、亲和层析及反向高效液相色谱操作，分析鉴定了棒纹牛蛭体内主要抗凝血物质成分为凝血酶抑制蛋白，其组成是三个相对分子质量分别为。7528，7446和7016的小分子量蛋白质，综合分析后认为属水蛭素类物质．  相似文献   

The spiral of silence: examining how cultural predispositions,news attention,and opinion congruency relate to opinion expression     

Shirley S. Ho  Vivian Hsueh-Hua Chen  Clarice C. Sim 《亚洲交流杂志》2013,23(2):113-134

This study examines new explanatory predictors behind the spiral of silence theory, using the issue of legalization of same-sex marriage in Singapore as the context of study. Our results show that fear of isolation and saving face were negatively associated with individuals' willingness to express their opinion on the issue, whereas news attention and issue salience were positively associated. Also, fear of isolation was negatively associated with individuals' willingness to offer a rationale for their opinion, whereas news attention and issue salience were positively associated. Power distance had no effects on outspokenness. Notably, news attention moderated the influence of fear of isolation and saving face on public outspokenness.  相似文献   

Social isolation and physical inactivity in older US adults: Results from the Third National Health and Nutrition Examination Survey     

《European Journal of Sport Science》2013,13(5):347-353

Abstract

Physical inactivity is a risk factor for cardiovascular and other chronic diseases. It has been shown that both physical inactivity and social isolation increase with age, and that these factors are detrimental to physical and mental well-being. The purpose of the present study was to examine the relationship between physical inactivity and social isolation in older US adults. Using data from a nationally representative cross-sectional survey, the Third National Health and Nutrition Examination Survey, 1988–1994, we assessed the age- and race-specific prevalence of no leisure-time physical activity in relation to various forms of social interaction. The prevalence of no leisure-time physical activity increases with age in both men and women. In non-Hispanic whites, non-Hispanic blacks, and Mexican Americans, the prevalence of no leisure-time physical activity is increased in older US adults who are socially isolated. Social isolation is related to physical inactivity among persons 60 years and older. Longitudinal and intervention studies are needed to establish whether increased social support translates to a more active population of older adults.  相似文献   

液-液隔离式毛细管黏度仪研制     

万桂怡  孙晓明  崔建军 《实验技术与管理》2013,30(6):36-38

黏度仪采用2个毛细管串联、中间设置一个液-液隔离器的研发方案。通过气动系统、智能控制系统等实现毛细管黏度计的精确测量。叙述了该系统的测量原理,介绍了该系统的结构和应用。  相似文献   

路基强度稳定技术研究   总被引：1，自引：0，他引：1  

张九香 《福建工程学院学报》2009,7(4)

由于路面竣工后,降水等入侵路基或地下水位发生变动时土基回弹模量值将大幅下降,引起路基强度失稳,进而损坏道路结构.文章分别研究分析了毛细水上升和气态水上升引起路基回弹模量的变化后路基强度降低对路面结构力学特性的影响程度,并基于此推荐采取隔断的办法以切断上下土层间的连接,阻断毛细水和气态水上升通道,以解决重载长效沥青路面中路基强度和稳定问题.  相似文献   

钢弹簧地铁浮置隔振装置的力学模型分析     

侯勇  张向慧 《北京工业职业技术学院学报》2009,8(1):34-36

对地铁浮置隔振装置建立了力学模型,并利用有限元分析软件对模型进行了力学分析,证实了浮置板轨道结构良好的隔振性,从而得出了浮置隔振装置在轨道上的最佳分布方案。  相似文献   

基于MSP430单片机的电机智能转速监测系统设计     

淮文军  石贵江  於汝彪 《苏州市职业大学学报》2012,23(1):15-18

设计一种由MSP430单片机构成的智能转速监测系统．该系统由与电机同轴的齿轮信号盘和霍尔转速传感器组成,对霍尔转速传感器输出的信号进行滤波和整形,使处理后的信号转换成标准的方波信号,隔离后由单片机计数,通过LED显示实时转速值．与传统的测速仪相比,可设定转速监测范围、存储设定参数,当电机超速时,可发出报警信号．试验证明,该系统测速精度高,电机状态监测可靠．  相似文献