Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing     

Qi WANG  Xiaoxia SHEN  Tian QIU  Wei WU  Lin LI  Zhi'an WANG  Huixia SHOU 《Journal of Zhejiang University. Science. B》2021,22(2):99

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.  相似文献   

构建基于数据挖掘技术的客户关系管理系统     

俞鸣春  黄江燕  夏小云 《科技广场》2005,(12):51-53

随着企业对客户关系管理的愈加重视，数据挖掘技术已成为实现客户关系管理的重要手段。本文介绍了客户关系管理系统和数据挖掘相关技术，并结合保险业的特点。分析了数据挖掘技术在保险CRM中的应用案例，指出了其商业价值。  相似文献   

论图书馆开放式读者服务的"场效应"   总被引：1，自引：0，他引：1  

霍仲常 《黔南民族师范学院学报》2004,24(4):96-99

开放式图书馆的读者服务是一种优质服务，同时又有一种“场效应”。这种“场效应”既具有主体教育功能，也能够培养主人翁(主体)精神，同时还能够培育平等和谐的服务关系。因此应该大力弘扬这种服务理念。  相似文献   

我国概念图研究概览   总被引：1，自引：0，他引：1  

张丽萍  吴淑花  何琪 《现代教育技术》2007,17(5):34-37

文章统计了1985.1—2005.6我国学者在概念图方面所发表论文的数目,分析了概念图研究的范域和成果,归纳了国人在概念图方面的研究特点与不足。  相似文献   

State Power,Symbolic Capital,and the Hierarchy and Homogeneity of Higher Education in China: In the Example of Three Key Universities Policies After the Founding of New China     

Li Xiaohong 《Chinese Education & Society》2019,52(3-4):208-230

Abstract

The structure of higher education in China is characterized by a high degree of hierarchy as well as strong homogeneity, differing from not only American higher education, which features a high degree of both hierarchy and heterogeneity, but also higher education in continental Europe, which exhibits a low degree of hierarchy. Previous studies have provided analysis of the structural characteristics of higher education in the United States, Europe, and elsewhere, as well as their differences, but have been unable to explain the situation in China. Drawing on Pierre Bourdieu’s field theory, this article proposes an explanatory model for the field of higher education as shaped by state power. The state created various forms of symbolic capital linked to economic capital in the field of higher education, and monopolized the quantity in which and means by which these are bestowed, thus causing differentiation in the total amount and composition of symbolic capital and economic capital between different schools, and forming a steeply stratified structure. The bestowal of symbolic capital was not restricted to a particular group of institutions of higher education: instead, the scope of this bestowal was gradually expanded, such that the vast majority of institutions of higher education regard the acquisition of symbolic capital and its attendant economic capital as the objective in their endeavors, resulting in the development of strong homogeneity between institutions. The article applies field theory to three key universities policies after the founding of New China, to describe and analyze the influence of symbolic capital on the field structure of higher education in China.  相似文献   

Adapting an Interprofessional Training Model for Social Work Field Placements: An Answer for Better Mental Health Care Outreach for Older Adults in Primary Care     

Avani Shah  Tracy Wharton  Forrest Scogin 《Journal of Teaching in Social Work》2017,37(5):438-453

Professional shortages of geriatrically trained social workers pose a barrier to mental health care for older adults. Integrating graduate social work interns into primary care settings may increase the availability of trained social workers. However, few studies provide guidance on how to develop an interprofessional healthcare placement focused on the needs of older adults. This article discusses a model training program created by a psychology department (with input from social work faculty) that can be adapted by schools of social work. Client outcomes (n = 47) indicate significant improvement when using this model.  相似文献   

关于有限环的若干结果     

陈建祥 《韩山师范学院学报》1994,(3)

环是近世代数的重要概念之一。本文主要讨论有限环是域的若干充分必要条件,揭示了这些条件可以作为有限整环、除环、域等重要概念的刻划。这将有助于我们进一步了解和研究有限环的构造。  相似文献   

从世界田径锦标赛看中国男子100 m的发展对策     

黄武平 《宁德师专学报(自然科学版)》2004,16(4):370-372,389

当前中国男子100m项目竞赛水平和世界前列水平存在较大差距．研究分析了中国男子100m比赛最好成绩和世界田径锦标赛成绩的差异，以期寻求缩小差距迎头赶上的对策．  相似文献   

最新的SOPC技术与EDA实验教学     

韦思健  张驰  韩文龙  马博坤 《实验技术与管理》2006,23(1):113-115

本文以清华大学科教仪器厂开发的TPG-EDA／SOPC教学实验平台为例介绍了传统的EDA（电子设计自动化）实验以及最新的SOPC（基于可编程片系统）技术，基于SOPC技术的系统级设计可以使用RISC（精简指令集）处理器软核，用来支持学生各种不同的实验项目。通过使用EDA设计工具，RISC软核处理器的C编泽器以及大量的FPGA（现场可编程门阵列）资源，可以使用或者重复使用IP来完成各种学生感兴趣的实验项目，培养和锻炼学生的系统级设计能力。  相似文献   

Technology student learning preferences and the design of flexible learning programs   总被引：1，自引：0，他引：1  

Peter J. Smith 《Instructional Science》2001,29(3):237-254

The learning preferences of three hundred and thirty eighttechnology students enrolled in sub-degree programs at an Australian institution of Technical andFurther Education were tested using the Canfield Learning Styles Inventory (CLSI). The results have been interpreted in a learning preferences framework and providesupportive evidence for the preferences factors of print-nonprint, collaborative, dependent,and autonomous learning identified by Sadler-Smith & Riding (1999). Although theresearch focussed on learning preferences the analysis also indicated support for theWholist-Analytic cognitive style proposed by Riding & Cheema (1991). Genderdifferences were shown for the Interest subscales of the CLSI. Age-group differenceswere shown for several Conditions of Learning and Modes of Learning subscales.Implications for the design of training programs, and the skillsthat may need to be developed in technology learners to enable effective use offlexible delivery, are also discussed.  相似文献