Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity   总被引：1，自引：0，他引：1  

韩明勇  郑树  于金明  彭佳萍  郭其森  王家林 《Journal of Zhejiang University. Science. B》2004,(4)

INTRODUCTIONInterleukin-18(IL-18),originallydescribedasinterferon(IFN)-g-inducingfactor,isan18.3-kDaproinflammatorycytokineproducedbyactivatedmacrophagesanddendriticcells,andplaysanim-portantroleintheTh1response,primarilybasedonitsabilitytoinduceIFN-gproductioninTcellsandNKcells.IL-18inducesproliferationofactivatedTcells,secretionofseveralcytokines,andpartici-patesinbothinnateandacquiredimmunity.TheroleofIL-18inantitumorimmunitywassuggestedinmanystudies.Genetransferofcytokinesisani…  相似文献   

人肝癌细胞总RNA电转染树突状细胞对T细胞的激活效应     

单铁英  苏安英  唐军民 《河北北方学院学报(医学版)》2008,25(5)

目的:采用Trizol一步法提取人肝癌细胞总RNA电转染人外周血树突状细胞(Dendritic Cell,DC),观察其对混合T淋巴细胞的体外激活效应。方法:通过密度梯度离心法分离人外周血单核细胞,用细胞因子体外培养诱导其成为DC。Trizol一步法提取人肝癌细胞总RNA。通过电转染法将人肝癌细胞总RNA导入DC内;通过混合淋巴细胞实验,获取DC激活的特异性效应T细胞。用MTT法测定效应T细胞的增殖率。结果:电转染方法可将人肝癌细胞总RNA导入DC;电转染前后DC分子表达无显著差异。转染了人肝癌细胞总RNA的DC可特异的激活T细胞且增殖率明显增强(P<0.05)。结论:电转染为人肝癌细胞总RNA导入DC提供技术上的可行性;转染了人肝癌细胞总RNA的DC可特异激活T细胞。  相似文献   

Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice     

Yong-fang Jiang  Jing Ma  Yan He  Yong-hong Zhang  Yun Xu  Guo-zhong Gong 《Journal of Zhejiang University. Science. B》2009,10(1):7-13

  相似文献   

CCR5稳定表达的CHO细胞的构建     

王芳宇  潘忠诚 《衡阳师范学院学报》2006,27(3):66-69

在人体内,CCR5与许多免疫疾病有关,CCR5有望成为众多药物的作用靶点。将ccr5基因与真核表达载体pBBS242构建成重组质粒pBBS242-ccr5,转染CHO细胞,并经潮霉素B筛选。流式细胞仪检测结果表明CCR5在CHO细胞得到了稳定表达。  相似文献   

EGFP基因转染骨髓间充质干细胞的实验研究     

郑立恒  魏会平  许松梅  何波  杨明理  王黎明  刘全胜 《河北北方学院学报(医学版)》2006,23(4):17-19

目的:探讨外源性EGFP基因转染修饰兔骨髓间充质干细胞(m esenchymal stem cells,MSCs)的可行性。方法:用增强型荧光绿色蛋白(enhanced green fluorescent protein,EGFP)作为报告基因,以脂质体法转染骨髓间充质干细胞,观察转染结果、表达情况及对靶细胞活力的影响。结果:经荧光显微镜下观察证实:成功地对骨髓间充质干细胞实现了基因转染。结论:兔骨髓间充质干细胞能够在体外适宜的条件下进行长期培养;骨髓间充质干细胞可直接作为基因靶细胞,建立稳定表达EGFP的骨髓间充质干细胞系具有可行性,为进一步做标记的MSCs细胞的移植研究奠定基础。  相似文献   

Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity     

韩明勇  郑树  于金明  彭佳萍  郭其森  王家林 《浙江大学学报(A卷英文版)》2004,5(4):472-476

To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhIL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hIL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3±4.5 pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine.  相似文献