共查询到18条相似文献，搜索用时 218 毫秒
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《南阳师范学院学报》2018,(3):21-25
利用ISSR标记对14个月季品种进行遗传多样性分析.正交设计确定20μL最佳ISSR-PCR反应体系,筛选7条引物对14个月季品种的基因组DNA进行ISSR-PCR扩增.7条引物共扩增出60条DNA条带,多态性位点52个,多态性位点百分率为86.0%,品种间遗传一致度为0.500.85.UPGMA聚类显示,藤本月季与大花月季和微型月季存在不同程度的亲缘关系.月季品种间的遗传多样性较高,大花月季、藤本月季和微型月季的品种类群间可能发生过杂交和基因交换. 相似文献
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《昆明师范高等专科学校学报》2014,(6)
为探明弥勒市8个主栽葡萄品种的亲缘关系,研究以这8个葡萄品种为材料,利用ISSR分子标记技术进行基因组多态性检测,从40条ISSR随机引物中筛选出10条用于ISSR扩增.结果显示,筛选出的这10条引物共扩增出81条谱带,其中66条为多态性谱带,多态性百分率较高,为79.9%;根据ISSR的扩增结果进行相似性系数分析,8个葡萄品种的遗传相似性系数在0.50~0.78之间,平均为0.647;通过UPGMA进行聚类分析,在遗传相似性系数为0.58处,8份试材分为2大类群,其中第1大类群包含3份欧美杂种品种和1份未知来源品种,第2大类群包含4份欧亚种品种.由此推测未知来源的这一品种属于欧美杂交种品种. 相似文献
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不同地区浒苔（Enteromorpha prolifera）群体遗传多样性的ISSR分析 总被引：3，自引：0，他引：3
通过ISSR分子标记技术对6个不同地区浒苔(Enteromorpha prolifera)群体(浙江鳌江、浙江宁波、广东南澳、江苏南通、福建福鼎、浙江南麂岛)进行了遗传多样性分析.结果显示,从100条ISSR引物中筛选出5条引物,共得到36个清晰的扩增位点,其中多态性位点10个;6个地区浒苔的多态位点比率为27.78%,平均杂合度为0.1256,Shannon's信息指数为0.1795;群体间的遗传距离在0.0870-0.2062之间,平均遗传距离为0.1654.研究表明,6个地区浒苔的遗传多样性处于较低水平,遗传变异相对贫乏;它们之间的亲缘关系与其地理距离的远近没有直接的关系,并揭示6个地区的浒苔是属于同一物种. 相似文献
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王子迎 《安徽教育学院学报》2005,23(6):94-96,105
采用随机扩增多态性DNA(RAPD)和简单序列重复区间扩增多态性(Inter-simple sequence repeat,ISSR)技术,利用12条RAPD引物和10条ISSR引物对10个安徽香菇菌株进行了DNA标记比较分析.结果表明,ISSR-PCR较RAPD-PCR稳定,更具可操作性;供试菌株存在比较显著的遗传变异,具有较丰富的遗传多样性. 相似文献
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为了对本课题组得到的大批优质棉花种质资源进行RAPD分子标记遗传多样性鉴定,我们进行了对棉花有效的引物对的筛选,并完成了24个优质棉花材料的遗传多样性分析.结果从48个随机引物对中筛选出了20对引物,这些引物对这24个优质棉花材料的有效性非常高,共扩增出113条谱带,平均每个引物扩增出5.65条,而且这20个随机引物对这24个材料的多态性谱带扩增效果非常明显,多态率高,能明显分辨出来源不同的材料.将这些多态性标记通过聚类软件分析,可将这24种材料明显的分为六类群,遗传多态性得到明显的体现. 相似文献
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胡桃楸ISSR-PCR反应体系的建立及优化 总被引：3，自引：0，他引：3
在研究胡桃楸遗传多样性的过程中,为了获得清晰、重复性好的ISSR扩增结果,采用改良的CTAB法提取胡桃楸基因组DNA,利用正交设计与单因子试验相结合的方法对影响胡桃楸ISSR-PCR反应体系的5个主要因素(Taq酶、Mg~(2+)、dNTP、引物、模板DNA)在4个水平上进行优化设计。通过综合比较分析,筛选出各反应因素的最佳水平,建立胡桃楸ISSR-PCR最佳反应体系:20μL的反应体系中,Taq酶1.0 U,Mg~(2+)2.0 mmol/L,dNTP 0.2 mmol/L,模板DNA 50～250 ng,引物0.4μmol/L。在此基础上筛选出13条多态性好、扩增稳定的ISSR引物,并确立了最佳退火温度和循环次数。这一优化系统的建立为今后利用ISSR技术进行胡桃楸遗传多样性、亲缘关系以及物种保护等的研究奠定了基础。 相似文献
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李勇慧 《洛阳师范学院学报》2024,(2):27-32
通过对河南25个居群的6种铁线莲种质进行遗传多样性分析,研究铁线莲种质资源分布和种群间的亲缘关系.筛选出6对引物,利用SSR标记技术对120个铁线莲样品进行遗传多样性分析.结果表明,25个居群的120个铁线莲的PIC值范围为0.619～0.738,有效等位基因数(Ne)为1.577±0.233,遗传相似系数在0.58到1.03之间,6对引物扩增出33条带,平均每个引物5.5条.同一种质的铁线莲优先聚为一类,同一种质亲缘关系更近,地理位置较近的亲缘关系也较近,25个居群的铁线莲具有丰富的遗传多样性.研究结果可为后续铁线莲种质创新和新品种选育提供参考. 相似文献
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《南阳师范学院学报》2019,(6):32-36
采用目标起始密码子多态性(SCoT)技术来分析16个月季品种的遗传多样性.通过对SCoT-PCR反应体系优化和PCR扩增程序探索,从55条SCoT分子标记引物中筛选出9条引物.品种间成对遗传相似度变幅介于0.400 0～0.775 0,9条SCoT标记引物的扩增多态性位点百分率为93.5%,显示月季品种间具有较丰富的遗传多样性.分析UPGMA聚类结果可知:16个月季品种在遗传相似性系数为0.62处被划分成2个聚类分支,第一分支又分为三个亚分支,显示不同月季品种间具有明显的遗传分歧,遗传关系复杂.大花月季、丰花月季与微型月季的亲缘关系相对较近,推测月季品种在驯化进程中存在基因重组和基因渐透现象;藤本月季进化独立,与其他园艺类群月季存在较明显的遗传分歧. 相似文献
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Manuel TORAL IBAEZ Margarita CARU Miguel A.HERRERA Luis GONZALEZ Luis M.MARTIN Jorge MIRANDA Rafael M.NAVARRO-CERRILLO 《Journal of Zhejiang University. Science. B》2009,10(2)
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin. 相似文献
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Manuel Toral Ibañez Margarita Caru Miguel A. Herrera Luis Gonzalez Luis M. Martin Jorge Miranda Rafael M. Navarro-Cerrillo 《Journal of Zhejiang University. Science. B》2009,10(2):112-119
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene
diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied.
The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.
The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis
in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and
were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index
and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).
Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of
49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic
variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a
geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although
they are derived from the same geographic origin.
Project (No. CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile 相似文献
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Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding 总被引：1，自引：0，他引：1
Jun-mei Wang Jian-ming Yang Jing-huan Zhu Qiao-jun Jia Yue-zhi Tao 《Journal of Zhejiang University. Science. B》2010,11(10):792-800
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR)
genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A
total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus
ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based
on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley
varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley
varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing
elite genotypes from different origins for malting barley breeding in China. 相似文献
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韩颖 《内江师范学院学报》2008,23(2):51-54
采用改进的CTAB法,成功地提取了北野豌豆及其近缘种的基因组DNA,电泳结果显示DNA质量和产量均较高,并以此DNA为模板,对ISSR的反应条件进行了优化,最终确定ISSR的反应体系为：总体积10μl,包括10ng的DNA,1倍的反应缓冲液,1．5mmol·L^-1MgCl2,2×10^-4mmol·L^-1（引物6和引物8）或2×10^-3mmol·L^-1（引物5,10和12）引物,0．2mmol·L^-1dNTP混合物和1．0U的Taq DNA聚合酶．ISSR扩增程序为：先在94℃下变性3min,然后在92℃下变性30s,50℃（引物5,6,10,12）或55．2℃（引物8）下复性30s,72℃下延伸1min,反应30个循环,最后72℃延伸5min．并基于此反应条件,最终筛选出5条ISSR引物用于进一步的研究． 相似文献
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Gabriel PEREZ Valentina VERDEJO Clarissa GONDIM-PORTO Julieta ORLANDO Margarita CARU 《Journal of Zhejiang University. Science. B》2014,15(11):966-978
研究目的：开发具有种属特异性序列特征性扩增区域（SCAR）标记物来监测哈茨木霉在其入侵的试验菌群中的定殖和生长,为哈茨木霉应用于生物防治等生态和生物技术中提供支撑。
创新要点：多种木霉属真菌能与各种微观和宏观的生物有机体建立相互作用。利用这些相互作用,木霉可做为原生种群的入侵物种而用于生物防治。本文通过使用试验菌群为研究模型,利用随机扩增多态性DNA（RAPD）技术和序列特征性扩增区域（SCAR）标记物来监测菌群中哈茨木霉的生长状态。
研究方法：利用随机扩增多态性DNA（RAPD）技术,从16个10聚体引物进行多态性筛选,其中1个引物扩增出对应哈茨木霉的条带。对该条带进行克隆测序,并设计5个20-23聚体引物。成功利用引物对2F2／2R2和2F2／2R3278分别特异性地扩增出哈茨木霉BpTloa菌株278bp和448bp的DNA片段。同时,用这两个引物对14个哈茨木霉菌株和几种不同的真菌菌株进行特异性对照试验,也只成功扩增出哈茨木霉菌株。此外,使用真菌DNA混合物和试验真菌群的DNA为模板,采用实时聚合酶链式反应（PCR）对引物对2F2／2R2进行评估。当仅使用100份SCAR标记物或哈茨木霉仅占整个菌群的0．1％时,仍能检测出哈茨木霉。
重要结论：本研究所建立的SCAR分子标记能有效监测菌群中的哈茨木霉的定殖和生长,具有较高特异性、灵敏度和准确度。 相似文献
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中国桫椤科6种植物的RAPD分析 总被引：8，自引：1，他引：8
应用随机扩增多态性DNA (RAPD)的方法分析中国桫椤科 6种植物 :小羽桫椤(Cyatheatsangii)、粗齿桫椤 (Alsophiladenticulata)、黑桫椤 (Alsophilapodophylla)、白桫椤(Sphaeropterisbrunoniana)、海南白桫椤 (Sphaeropterisbrunoniana)、桫椤 (Alsophilaspinulosa)。经筛选 4 0个引物 ,发现 14个引物的带型清晰并呈多态性。采用UPGMA法对求出的遗传距离进行聚类分析 ,得到的结果显示 :所研究 6种植物明显地聚合为 3个类群 ,支持夏群的分类处理 相似文献
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通过查阅标本,实地考察,参考有关文献,对甘肃省委陵菜属植物进行了研究,整理出甘肃省有委陵菜属植物52种及变种,编制了甘肃委陵菜属植物检索表,考证了其学名,记述了它们的分布地及生境,对部分种及其相近种进行了分类学讨论,以期为甘肃委陵菜属植物的基础研究和开发利用提供本底资料. 相似文献