Protective Role of <Emphasis Type="SmallCaps">l</Emphasis>-Arginine Against Free-Radical Mediated Oxidative Damage in Patients with Unstable Angina     

Tripathi P  Chandra M  Misra MK 《Indian journal of clinical biochemistry : IJCB》2010,25(3):302-306

Unstable angina is a critical condition of heart resulting from narrowing of vessels supplying blood to heart. Ischemia of the myocardium leads to oxidative stress and severe tissue damage. The objective of the present study was to determine the effect of l-arginine administration on the oxidant–antioxidant homeostasis which otherwise gets imbalanced in patients with cardiovascular diseases. The results obtained, show improvement in the oxidant–antioxidant levels of the subjects upon incorporation of l-arginine. Our findings suggest that supplementation of l-arginine along with regular anti-anginal therapy may be beneficial to the patients of unstable angina.  相似文献   

海带多酚提取工艺优化及抗氧化性研究     

汪群  雷思佳  付慧娟  林珊  郑康燕  赖雯娟  周如意 《深圳职业技术学院学报》2021,(1)

以海带多酚为研究对象,考察提取温度、料液比和超声时间对海带游离多酚和结合多酚提取率的影响、采用正交试验对多酚提取工艺进行优化,并通过测定多酚对DPPH自由基的清除能力评估其抗氧化性.结果表明,海带多酚的最佳提取工艺为:料液比为1:30(g/mL)、提取温度为40℃、超声时间为30 min.在此工艺条件下,海带总多酚的提取率为2.74%.当浓度为2.0 mg/mL时,游离多酚和结合多酚对DPPH·的清除率分别为41.72%和19.37%,表明海带多酚具有一定的抗氧化活性,且游离多酚的抗氧化活性高于结合多酚。  相似文献   

Role of Oxidative Stress in Various Stages of Psoriasis     

Kadam DP  Suryakar AN  Ankush RD  Kadam CY  Deshpande KH 《Indian journal of clinical biochemistry : IJCB》2010,25(4):388-392

Psoriasis is a chronic inflammatory, proliferative skin disease characterized by pathological skin lesions due to various exogenous and endogenous factors. It is associated with a number of biochemical and immunological disturbances. Recently, it has been suggested that increased reactive oxygen species (ROS) production and compromised function of antioxidant system may be involved in the pathogenesis of this disease. In the present study, 90 psoriasis patients were selected. Disease severity was assessed by psoriasis area severity index score and grouped as mild, moderate and severe (each group consists of 30 subjects) and compared with 30 healthy controls. Serum levels of malondialdehyde, nitric oxide end products and the activities of antioxidant enzymes such as erythrocyte-superoxide dismutase, catalase and total antioxidant status were investigated in these groups/subjects. As compared to controls, we found severitywise significantly increased serum malondialdehyde, nitric oxide end products with decrease in erythrocyte-superoxide dismutase activity, catalase activity and total antioxidant status in patients with psoriasis suggesting worsening of the disease. It seems to be linked with the enhancement of Reactive Oxygen Species production and decreased antioxidant potential in psoriasis.  相似文献   

Oxidative injury and antioxidant vitamins E and C in Schizophrenia     

Benedicta D'Souza  Vivian D'Souza 《Indian journal of clinical biochemistry : IJCB》2003,18(1):87-90

Susceptibility of Schizophrenic patients to lipid peroxidation relative to healthy control subjects was investigated by measuring the malondialdehyde (MDA) levels in plasma. The main finding was that Schizophrenic patients were more susceptible than control subjects to oxidative damage as evident from increased MDA levels in plasma. Antioxidant levels are also depleted in Schizophrenic patients when compared to normal subjects as evident from decreased levels of vitamins E and C in the plasma. Impaired antioxidant defense and increased lipid peroxidation suggests that treatment with antioxidants (Vitamin E, Vitamin C, beta carotene) at the initial stages of illness may prevent further oxidative injury and deterioration of associated neurological deficits in Schizophrenia.  相似文献   

Lipid peroxide levels and antioxidant status in alcoholic liver disease     

Seema Gupta  Rajesh Pandey  Ranjan Katyal  H. K. Aggarwal  R. P. Aggarwal  S. K. Aggarwal 《Indian journal of clinical biochemistry : IJCB》2005,20(1):67-71

The present study was conducted to evaluate some of the components of antioxidant defense system and oxidative damage in 20 male patients of alcoholic liver disease (ALD). The results were compared with 20 healthy male smokers and 20 healthy male non-smokers volunteers. Patients were subjected to detailed clinical examination and laboratory investigations. Blood samples were collected for estimating reduced glutathione (GSH), total thiols (T-SH) malondialdehyde (MDA), transaminases (AST, ALT), glutathione-S-transferease (GST) and gammaglutamyl transferase (GGT). Serum aspartate amino transferase (AST)/alanine amino transferase (ALT) ratio was significantly (p<0.01) reduced in ALD patients as compared to the controls. However, the core of utility of MDA and GST was found to be significantly (p<0.01) increased in ALD patients compared to controls. There was a significant negative correlation of MDA with both GSH and TSH. Plasma GGT levels were significantly (p<0.01) increased in alcoholics and the enzyme showed a significant positive correlation with MDA. These results give enough evidence of increased oxidative stress and compromised antioxidant defense system in patients with ALD.  相似文献   

Risk factors for coronary heart disease in type II diabetes mellitus     

H. Surekha Rani  G. Madhavi  V. Ramachandra Rao  B. K. Sahay  A. Jyothy 《Indian journal of clinical biochemistry : IJCB》2005,20(2):75-80

Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. An attempt has been made to evaluate the risk factors for coronary heart disease in type II diabetics. In the present study the levels of fasting and postprandial plasma glucose, total cholesterol, low density lipoproteins, triglycerides were high and the levels of high density lipoproteins were low in the type II diabetics compared to controls. The markers of free radical induced injury i.e. malondialdehyde and nitrite/nitrate were high while total antioxidant status a marker for antioxidant protection against reactive oxygen species was low in diabetics compared to controls. The study therefore suggests the importance of assessing these markers of oxidative stress and antioxidant capacity along with the other routine investigations in diabetic patients for initiating antioxidant therapy in addition to primary and secondary preventive measures to mitigate the devastating consequences of diabetes leading to coronary heart disease.  相似文献   

Cardioprotective activity of synthetic guggulsterone (E and Z-isomers) in isoproterenol induced myocardial ischemia in rats: A comparative study     

Ramesh Chander  Farhan Rizvi  A. K Khanna  Ram Pratap 《Indian journal of clinical biochemistry : IJCB》2003,18(2):71-79

Guggulsterone, a mixture of cis (E) and trans (Z) isomers (7∶3 w/w) was synthesized from 16-DPA. The isomers were separated by column chromatography and evaluated for cardioprotective and antioxidant activities. Myocardial necrosis induced by isoproterenol in rats caused marked increase in serum creatine phosphokinase and glutamate pyruvate transaminase. Simultaneously in ischemic heart, phospholipase, xanthine oxidase and lipid peroxides were enhanced following depletion of glycogen, phospholipids and cholesterol. Treatment with guggulsterone and its both isomers at the dose of 50 mg/kg po., significantly protected cardiac damage as assessed by the reversal of blood and heart biochemical parameters in ischemic rats. The cardioprotective activity of guggulsterone and of both the isomers were compared with that of gemfibrozil at the same doses. Guggulsterone and both the isomers at tested concentrations (5–20mM) inhibited oxidative degradation of lipids in human low-density lipoprotein and rat liver microsomes induced by metal ionsin vitro. The drug counteracted against the generation of superoxide anions (O₂) and hydroxyl radicals (OH⁻) in non-enzymic test systems. It is suggested that cardioprotective and antioxidant activities of synthetic guggulsterone and guggulsterone obtained from gum resinCommiphora mukul that contains isomers E & Z in the ratio of 46∶54w/w are the same.  相似文献   

补充扇贝肽对运动大鼠停训后萎缩骨骼肌细胞Caspase-3活性的影响     

潘小玲  郭振新  庞阳康 《山东体育学院学报》2011,27(7):53-57

目的:通过测试萎缩骨骼肌细胞Caspase-3、SOD活性和MDA含量,观察补充扇贝肽对运动大鼠停训导致骨骼肌萎缩后肌细胞Caspase-3活性及其抗氧化能力的影响,研究扇贝肽对停训后骨骼肌萎缩的干预作用。方法:40只SD雄性大鼠以15 m/min的速度,持续性水平跑台训练30 min开始进行递增负荷运动,运动6 d/周,休息1 d/周,速度递增3 m/min/周,时间递增30 min/周,运动3周后即刻处死10只大鼠为基础对照组,其余大鼠随机分组为补充安慰剂阴性对照组、补充牛乳阳性对照组和补充扇贝肽实验组,30只大鼠左侧后肢实施石膏固定模拟停训模型,并进行扇贝肽补充实验。实验组和对照组所有动物每天分别进行灌胃15%扇贝肽饮料2mL、等量的安慰剂和牛乳。结果:发现补充扇贝肽可显著减低停训后萎缩骨骼肌细胞MDA含量增加,增加SOD活性,提高细胞内抗氧化能力,可显著抑制停训后萎缩骨骼肌细胞Caspase-3的活性,从而抑制骨骼肌收缩蛋白和结构蛋白的降解,促进萎缩骨骼肌的恢复。  相似文献   

黄芪总苷对运动性疲劳大鼠骨骼肌抗氧化能力、ATP酶活性及乳酸含量的影响     

李爽  艾英伟  阿拉木斯  王蕾 《吉林体育学院学报》2010,26(3):68-69

探讨黄芪总苷对运动性疲劳大鼠骨骼肌抗氧化能力、ATP酶活性及乳酸含量的影响。方法：应用力竭性游泳方法制作运动性疲劳大鼠模型,对大鼠预防性给予黄芪总苷,观察大鼠骨骼肌抗氧化能力ATP酶活性及乳酸含量的变化。结果：黄芪总苷明显提高运动性疲劳大鼠骨骼肌的POD,T-AOC和Ca^2＋Mg^2＋-ATPase的活性,降低LD的含量（P〈0.05）。结论：黄芪总苷具有保护运动性疲劳大鼠肌组织的作用,其机制可能与提高骨骼肌抗氧化能力,改善肌肉组织能量代谢有关。  相似文献   

骨骼肌抗氧化作用的信号转导机制   总被引：1，自引：0，他引：1  

赵永军  卢健 《吉林体育学院学报》2010,26(5):89-91

现已证明骨骼肌收缩诱导产生的活性氧（ROS）可造成骨骼肌氧化应激水平升高,同时肌肉的抗氧化防御系统的应答性也升高。而NFκB和MAPK是两条主要的氧化应激敏感性信号转导通路,可激活许多酶和蛋白质的基因表达,而这些酶和蛋白质在维持细胞内氧化还原稳态中发挥重要作用。综述讨论上述两条信号转导通路及其适应的生物学差异。  相似文献